中国寄生虫学与寄生虫病杂志 ›› 1984, Vol. 2 ›› Issue (3): 173-175.

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免疫荧光环卵沉淀反应诊断日本血吸虫病的研究

杨兆莘,蒋美荣,陈树仁,李家泉,江宝玲   

  1. 安徽医学院寄生虫学教研组
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1984-08-31 发布日期:2017-01-12

IMMUNOFLUOHESCENCE CIRCUMOVAL PRECIPITIN TEST IN THE DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA

  • Received:2017-01-12 Revised:2017-01-12 Online:1984-08-31 Published:2017-01-12

摘要: 在粪检阳性率为74.4%的血吸虫病重流行区,如以环沉率≥1%为阳性反应,则免疫荧光环卵沉淀反应(IFCOP)和常规环卵沉淀反应(COP)与粪检阳性的符合率均在90%以上;环沉率≥6%者的符合率均为69.5%,无显著性差异。粪检阳性率为15.4%的轻流行区如以环沉率≥1%为阳性反应,则两种方法与粪检阳性的符合率分别为88.9%和6.7%,差异显著:环沉率≥5%者,分别为39.7%和41.3%。提示在轻流行区IFCOP对抗体水平低的患者,其敏感性要高于常规COP,并表明若以环沉率≥5%为阳性界限,两法均有相当多的病人被漏诊。

关键词: 日本血吸虫病, 环卵沉淀反应, 环沉率, 免疫荧光, 血吸虫病防治, 阳性率, 阳性符合率, 重流行区, 寄生虫学, 流行区人群

Abstract: A comparative study on the diagnostic value of immunofluorescence technic(IFCOP) and the conventional COP test using heat-treated and ultrasonicated dry eggs of Schisto soma japonicum as antigens for the diagnosis of schistosomiasis japonica was carried out in areas of different endemicity of schistosomiasis japonica. Results showed no significant difference in sensitivity between two methods in the area of high prevalence. However, in the area of low prevalence IFCOP technic was significantly more sensitive than COP test. Results of tests on sera from 63 patients of schistosomiasis showed that IFCOP and COP were positive in 88.9% and 66.7% respectively. The results indicate that the sensitivity of the COP test is much enhanced by the fluorescent antibody technique in that the small circumoval precipitates can be detected more easily.