芜湖市区宠物犬、猫刚地弓形虫感染情况调查

doi:10.12140/j.issn.1000-7423.2022.01.020

中国寄生虫学与寄生虫病杂志 ›› 2022, Vol. 40 ›› Issue (1): 124-126.doi: 10.12140/j.issn.1000-7423.2022.01.020

芜湖市区宠物犬、猫刚地弓形虫感染情况调查

蒋峰¹, 陈润¹, 都宁宁¹, 朱梦怡¹, 钟昊¹, 陈辉¹, 奚旭霞², 湛孝东¹, 李朝品¹^,^*()

1 皖南医学院医学寄生虫学教研室,芜湖 241002
2 芜湖市第一人民医院妇产科,芜湖 241002

收稿日期:2021-05-19 修回日期:2021-07-06 出版日期:2022-02-28 发布日期:2022-01-07
通讯作者: 李朝品
基金资助:
国家自然科学基金(81270091);安徽高校自然科学研究重点项目(KJ2019A0403)

Investigation of Toxoplasma gondii infection in pet dogs and cats in Wuhu City

JIANG Feng¹, CHEN Run¹, DU Ning-ning¹, ZHU Meng-yi¹, ZHONG Hao¹, CHEN Hui¹, XI Xu-xia², ZHAN Xiao-dong¹, LI Chao-pin¹^,^*()

1 Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, China
2 Department of Obstetrics and Gynecology, the First People’s Hospital of Wuhu, Wuhu 241002, China;

Received:2021-05-19 Revised:2021-07-06 Online:2022-02-28 Published:2022-01-07
Contact: LI Chao-pin
Supported by:
National Natural Science Foundation of China(81270091);Key Project of Natural Science Research in Universities of Anhui Province(KJ2019A0403)

摘要/Abstract

摘要：

为了解芜湖市区宠物犬、猫刚地弓形虫感染情况,于2019年5—10月在芜湖市某宠物医院采集宠物犬、猫血样,应用环介导等温扩增（LAMP）技术检测宠物犬、猫弓形虫感染情况。共采集500份宠物犬、猫血样,阳性血样26份,阳性率为5.2%。宠物犬、猫阳性率分别为2.8%（7/250）和7.6%（19/250）,二者差异有统计学意义（χ² = 5.842,P < 0.05）。雄性和雌性宠物犬的阳性率分别为2.7%（4/147）和2.9%（3/103）,齿龄 ≤ 3和 > 3的阳性率分别为2.4%（4/166）和3.6%（3/84）,差异均无统计学意义（χ² = 0.000、0.014,P > 0.05）。雄性和雌性宠物猫的阳性率分别为8.3%（10/120）和6.9%（9/130）,差异无统计学意义（χ² = 1.177,P > 0.05）,齿龄 ≤ 3和 > 3的阳性率分别为6.5%（12/185）和10.8%（7/65）,差异有统计学意义（χ² = 1.256,P < 0.05）。提示芜湖市区宠物犬、猫弓形虫感染率较高,存在感染人的可能。

关键词: 刚地弓形虫, 环介导等温扩增, 犬, 猫, 芜湖市

Abstract:

Peripheral blood samples were collected from pet dogs and cats at a veterinary clinic in Wuhu from May to October, 2019, to investigate the prevalence of Toxoplasma gondii in pet dogs and cats in Wuhu urban area. Loop-mediated isothermal amplification (LAMP) technology was used to detect Toxoplasma gondii in pet dog and cat samples. T. gondii were detected from 26 of the 500 samples. The overall positive rate is 5.2% with 2.8% (7/250) for dogs, and 7.6% (19/250) for cats, respectively, the difference was statistically significant (χ ² = 5.842, P < 0.05). The positive rate in male and female pet dogs was 2.7% (4/147) and 2.9% (3/103), respectively, the positive rates in younger pet dogs (age ≤ 3 years) and elder dogs (age > 3 years) were 2.4% (4/166) and 3.6% (3/84), respectively, with no statistically significant difference (χ ² = 0.000, 0.014, P > 0.05). The positive rates in male and female cats were 8.3% (10/120) and 6.9% (9/130), respectively, with no statistically significant difference (χ ² = 1.177, P > 0.05). The positive rates in younger pets (age ≤ 3 years) and elder pets (age > 3 years) were 6.5% (12/185) and 10.8% (7/65), respectively. The difference was statistically significant (χ ² = 1.256, P < 0.05). The results suggested a relatively high prevalence of T. gondii in dogs and cats in Wuhu urban area, which may potentially infect human.

Key words: Toxoplasma gondii, Loop-mediated isothermal amplification, Dog, Cat, Wuhu City

中图分类号:

R531.8

蒋峰, 陈润, 都宁宁, 朱梦怡, 钟昊, 陈辉, 奚旭霞, 湛孝东, 李朝品. 芜湖市区宠物犬、猫刚地弓形虫感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(1): 124-126.

JIANG Feng, CHEN Run, DU Ning-ning, ZHU Meng-yi, ZHONG Hao, CHEN Hui, XI Xu-xia, ZHAN Xiao-dong, LI Chao-pin. Investigation of Toxoplasma gondii infection in pet dogs and cats in Wuhu City[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2022, 40(1): 124-126.

参考文献 16

[1]	Rajapakse S, Weeratunga P, Rodrigo C, et al. Prophylaxis of human toxoplasmosis: a systematic review[J]. Pathog Glob Health, 2017, 111(7): 333-342. doi: 10.1080/20477724.2017.1370528 pmid: 28948861
[2]	Su HY, Yang SJ, Peng HJ, et al. Current status of toxoplasmosis misdiagnosis in clinic[J]. Chin J Parasitol Parasit Dis, 2019, 37(3): 342-345. (in Chinese)
	(苏海莹, 杨淑君, 彭鸿娟, 等. 弓形虫病临床误诊现状分析[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(3): 342-345.)
[3]	Rouatbi M, Amairia S, Amdouni Y, et al. Toxoplasma gondii infection and toxoplasmosis in North Africa: a review[J]. Parasite, 2019, 26: 6. doi: 10.1051/parasite/2019006 pmid: 30767889
[4]	Dantas-Torres F, Otranto D. Dogs, cats, parasites, and humans in Brazil: opening the black box[J]. Parasit Vectors, 2014, 7: 22. doi: 10.1186/1756-3305-7-22 pmid: 24423244
[5]	Hernández-Cortazar I, Acosta-Viana KY, Ortega-Pacheco A, et al. Toxoplasmosis in Mexico: epidemiological situation in humans and animals[J]. Rev Inst Med Trop Sao Paulo, 2015, 57(2): 93-103. doi: 10.1590/S0036-46652015000200001
[6]	Cong W, Elsheikha HM, Zhou N, et al. Prevalence of antibodies against Toxoplasma gondii in pets and their owners in Shandong Province, Eastern China[J]. BMC Infect Dis, 2018, 18(1): 430. doi: 10.1186/s12879-018-3307-2 pmid: 30157863
[7]	Elsheikha HM. Congenital toxoplasmosis: priorities for further health promotion action[J]. Public Health, 2008, 122(4): 335-353. pmid: 17964621
[8]	Qian WF, Yan WC, Wang TQ, et al. Prevalence and genetic characterization of Toxoplasma gondii in pet dogs in central China[J]. Korean J Parasitol, 2015, 53(1): 125-128. doi: 10.3347/kjp.2015.53.1.125
[9]	Li YN, Nie X, Peng QY, et al. Seroprevalence and genotype of Toxoplasma gondii in pigs, dogs and cats from Guizhou Province, Southwest China[J]. Parasit Vectors, 2015, 8: 214. doi: 10.1186/s13071-015-0809-2
[10]	Hou ZF, Su SJ, Liu DD, et al. Prevalence, risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu Province, Eastern China[J]. Infect Genet Evol, 2018, 60: 17-25. doi: 10.1016/j.meegid.2018.02.007
[11]	Qiu HY. Investigation on the prevalence of dog and cat zoonotic parasites and analysis of risk factors in Northeast[D]. Daqing: Heilongjiang Bayi Agricultural University, 2020: 13-24. (in Chinese)
	(邱鸿宇. 东北地区犬、猫人兽共患寄生虫的流行情况调查及风险因素分析[D]. 大庆: 黑龙江八一农垦大学, 2020: 13-24.)
[12]	Wang Q, Jiang W, Chen YJ, et al. Prevalence of Toxoplasma gondii antibodies, circulating antigens and DNA in stray cats in Shanghai, China[J]. Parasit Vectors, 2012, 5: 190. doi: 10.1186/1756-3305-5-190
[13]	Sheng Z, Jin Y, Yao Y, et al. Seroprevalence of Toxoplasma gondii infection in pet dogs in Anhui Province, China[J]. Iran J Parasitol, 2020, 15(3): 446-451.
[14]	Watanabe M, Sadiq MB, Mulop NIA, et al. Prevalence of Toxoplasma gondii antibodies in stray dogs from various locations in west and east Malaysia[J]. Korean J Parasitol, 2020, 58(5): 487-492. doi: 10.3347/kjp.2020.58.5.487
[15]	Gao YM, Ding H, Lamberton PHL, et al. Prevalence of Toxoplasma gondii in pet dogs in mainland China: a meta-analysis[J]. Vet Parasitol, 2016, 229: 126-130. doi: 10.1016/j.vetpar.2016.10.009
[16]	Wang S, Zhou Y, Niu J, et al. Seroprevalence of Toxoplasma gondii infection in domestic cats in central China[J]. Parasite, 2017, 24: 10. doi: 10.1051/parasite/2017010

芜湖市区宠物犬、猫刚地弓形虫感染情况调查

Investigation of Toxoplasma gondii infection in pet dogs and cats in Wuhu City

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 16

相关文章 15

编辑推荐

Metrics

本文评价

[1]	贾西帅, 周水茂, 罗华堂, 刘聪, 王帅, 徐文秀. 利什曼原虫环介导等温扩增检测方法的建立及应用[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(3): 413-417.
[2]	姜宁, 白洁, 李平, 单文琪, 周秋明, 董昊炜, 袁浩, 陶峰, 李翔宇, 马雅军, 彭恒. 基于环介导等温扩增微流控芯片技术快速检测蚊媒携带登革病毒方法的建立[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(2): 234-241.
[3]	解晓曼, 孙航, 代莉莎, 朱文菊, 王利磊, 谢环环, 董宏杰, 张俊梅, 王琦, 周贝贝, 赵桂华, 徐超, 尹昆. 刚地弓形虫感染对小鼠脑组织转录本m⁶A甲基化修饰的影响[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 27-35.
[4]	郑广福, 刘现兵, 姜昱竹, 李新雨, 胡雪梅, 张海霞. 刚地弓形虫感染孕鼠胎盘组织中中性粒细胞和IL-17与不良妊娠结局的关系[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 48-54.
[5]	周水茂, 贾西帅, 杨燕, 张娟. 卫氏并殖吸虫环介导等温扩增检测方法的建立[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(1): 121-124.
[6]	李逢时, 赵姗姗, 谭文波, 吾热力哈孜·哈孜汗, 谷新利, 王素文, 刘钢, 王远志. 新疆红狐体表犬硬蜱的分子生物学和雄蜱形态学研究[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(6): 739-743.
[7]	薛羽珊, 林萍, 程训佳, 冯萌. 慢性弓形虫感染对宿主中枢神经系统的损伤及其作用机制[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 527-531.
[8]	姜文静, 孟雅莉, 赵利娜, 王春苗, 张晓磊. 刚地弓形虫棒状体蛋白18和膜表面抗原30复合核酸疫苗对小鼠的免疫保护作用[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(5): 532-538.
[9]	蔡长煌, 张芝平, 卓鸣莺, 郭理平, 傅志辉, 刘亦若. 基于虫卵内转录间隔区2序列分析诊断麝猫后睾吸虫和华支睾吸虫感染[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 427-433.
[10]	赵紫琪, 吕芳丽. 蒿甲醚脂质体体外抑制刚地弓形虫增殖作用的研究[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 446-451.
[11]	郑玉华, 帖萍, 白永飞, 闫昌福, 王婷, 王晶莹, 田晓东, 代培芳. 2021—2022年山西省内脏利什曼病流行区家犬感染情况及白蛉密度调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(4): 470-475.
[12]	张驰, 陈嘉婷, 辛紫萱, 杨莉莉, 杨梓瀚, 彭鸿娟. 弓形虫慢性感染小鼠脑转录组分析及与抑郁相关的犬尿氨酸通路的验证[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 270-278.
[13]	谢宜, 王莹, 王旭, 施丹丹, 付梅花, 李春阳, 伍卫平, 丹巴泽里, 廖沙, 张凯歌, 邓雪莹, 官亚宜. 基于高通量测序的家犬粪便寄生虫病原调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 325-330.
[14]	杜鹃, 李佳, 吴迪, 余琦, 张玮, 白如念, 郭俊林, 刘庆斌, 雷琪莉, 谷传慧, 王萌, 赵浩军. 2022年北京市犬猫刚地弓形虫感染血清流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 389-392.
[15]	李永霞, 李梦蕊, 李诗艺, 黄潇航, 吴炜, 黄志坚, 殷光文. 福建漳州流浪犬、猫芽囊原虫感染调查及基因分型[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(2): 245-248.