上转发光免疫层析法快速检测棘球蚴病患者血清IgG的评价

doi:10.12140/j.issn.1000-7423.2022.01.019

中国寄生虫学与寄生虫病杂志 ›› 2022, Vol. 40 ›› Issue (1): 121-123.doi: 10.12140/j.issn.1000-7423.2022.01.019

上转发光免疫层析法快速检测棘球蚴病患者血清IgG的评价

刘佳¹(), 马俊英¹^,^*(), 张雪飞¹, 王虎², 马霄¹, 蔡辉霞¹, 高琦³, 郄霜³, 尹艳³, 王威¹, 王永顺¹, 张静宵¹, 刘玉芳¹, 雷雯¹, 詹培珍¹, 张青¹

1 青海省地方病预防控制所,西宁 811602
2 青海省卫生健康委员会,西宁 810000
3 北京热景生物技术股份有限公司,北京 100000

收稿日期:2021-07-27 修回日期:2021-11-16 出版日期:2022-02-28 发布日期:2022-01-30
通讯作者: 马俊英
作者简介:刘佳（1989-）,女,硕士,主管医师,主要从事寄生虫病防治研究。E-mail： 457831207@qq.com
基金资助:
青海省2020年科技计划项目(2020-SF-133)

Evaluation of up-converting phosphor-lateral flow method for rapid detection of IgG antibodies in echinococcosis patients

LIU Jia¹(), MA Jun-ying¹^,^*(), ZHANG Xue-fei¹, WANG Hu², MA Xiao¹, CAI Hui-xia¹, GAO Qi³, QIE Shuang³, YIN Yan³, WANG Wei¹, WANG Yong-shun¹, ZHANG Jing-xiao¹, LIU Yu-fang¹, LEI Wen¹, ZHAN Pei-zhen¹, ZHANG Qing¹

1 Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, China
2 Health Commission of Qinghai Province, Xining 810000, China
3 Beijing Hotgen Biotechnology Co., Ltd, Beijing 100000, China

Received:2021-07-27 Revised:2021-11-16 Online:2022-02-28 Published:2022-01-30
Contact: MA Jun-ying
Supported by:
2020 Science and Technology Plan Project of Qinghai Province(2020-SF-133)

摘要/Abstract

摘要：

初步评价上转发光免疫层析（UPT-LF）法快速检测棘球蚴病患者血清IgG的效果,并与酶联免疫吸附试验（ELISA）法检测结果作比较。2017—2018年,从青海省人民医院和青海大学附属医院收集棘球蚴病临床确诊患者血清,同时从各医院体检中心收集健康者血清,以UPT-LF法和ELISA法检测血样中棘球蚴IgG抗体,以临床诊断结果为标准分析两种检测方法检测的灵敏度、特异度、误诊率、总符合率、约登指数等。两种检测结果的比较进行卡方检验。共收集棘球蚴病患者血样104份,其中多房棘球蚴病（AE）46份,细粒棘球蚴病（CE）58份;非棘球蚴病患者血样165份。UPT-LF、ELISA法检测104份临床确诊棘球蚴病患者血清的灵敏度分别为94.2%（98/104）、78.8%（82/104）,二者差异有统计学意义（χ² = 11.250,P < 0.01）,其中检测AE的灵敏度分别为97.8%（45/46）、89.1%（41/46）,检测CE的灵敏度分别为91.4%（53/58）、70.7%（41/58）;UPT-LF、ELISA法检测165份非棘球蚴病患者血清的特异度分别为95.2%（157/165）、90.9%（150/165）,二者差异无统计学意义（χ² = 1.565,P > 0.05）。UPT-LF、ELISA法检测结果与临床诊断结果的符合率分别为94.8%（255/269）、86.2%（232/269）,二者差异无统计学意义（χ² = 1.884,P > 0.05）。UPT-LF、ELISA法的约登指数分别为0.894、0.697。与ELISA法相比,UPT-LF法的灵敏度更高,具有良好的棘球蚴病现场快速检测应用前景。

关键词: 棘球蚴病, 上转发光免疫层析, 快速检测

Abstract:

To evaluate the effect of up-converting phosphor-lateral flow (UPT-LF) method for rapid detection of IgG antibodies in echinococcosis patient, and compare the test results with that of enzyme-linked immunosorbent assay (ELISA) method. Serum samples from patients with clinically confirmed echinococcosis were collected at Qinghai Provincial People’s Hospital and the Affiliated Hospital of Qinghai University from 2017 to 2018. Serum samples of healthy individuals who came for general healthy checks were also collected from the physical examination centers of those hospitals. The anti-echinococcosis IgG antibody in the samples was detected by UPT-LF and ELISA methods. The sensitivity, specificity, misdiagnosis rate, total coincidence rate and Yoden index were analyzed based on clinical diagnosis results. Chi-square test was performed to compare the results between the two methods. A total of 104 serum samples of echinococcosis patients, including 46 cases of alveolaris echinococcosis (AE), 58 cases of cyst echinococcosis (CE) and 165 serum samples from healthy individuals were collected. The sensitivity of UPT-LF and ELISA in echinococcosis patients were 94.2% (98/104) and 78.8% (82/104), respectively, with statistically significant difference between the two methods (χ² = 11.250, P < 0.01). The sensitivity of UPT-LF and ELISA in AE patients were 97.8% (45/46) and 89.1% (41/46), while 91.4% (53/58) and 70.7% (41/58) in CE patients, respectively. The specificity of UPT-LF and ELISA in non-echinococcosis patients were 95.2% (157/165) and 90.9% (150/165), respectively, with no statistically significant difference between the two methods (χ ² = 1.565, P > 0.05). Compared with the clinical diagnosis result, the coincidence rates of UPT-LF and ELISA were 94.8% (255/269) and 86.2% (232/269), respectively, with no statistically significant difference between the two methods (χ ² = 1.884, P > 0.05). The Youden index of UPT-LF and ELISA were 0.894 and 0.697, respectively. UPT-LF method showed higher sensitivity than that of ELISA, and has a good application prospect in the field for the rapid detection of echinococcosis.

Key words: Echinococcosis, Up-converting phosphor-lateral flow, Rapid detection

中图分类号:

R532.32

刘佳, 马俊英, 张雪飞, 王虎, 马霄, 蔡辉霞, 高琦, 郄霜, 尹艳, 王威, 王永顺, 张静宵, 刘玉芳, 雷雯, 詹培珍, 张青. 上转发光免疫层析法快速检测棘球蚴病患者血清IgG的评价[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(1): 121-123.

LIU Jia, MA Jun-ying, ZHANG Xue-fei, WANG Hu, MA Xiao, CAI Hui-xia, GAO Qi, QIE Shuang, YIN Yan, WANG Wei, WANG Yong-shun, ZHANG Jing-xiao, LIU Yu-fang, LEI Wen, ZHAN Pei-zhen, ZHANG Qing. Evaluation of up-converting phosphor-lateral flow method for rapid detection of IgG antibodies in echinococcosis patients[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2022, 40(1): 121-123.

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上转发光免疫层析法快速检测棘球蚴病患者血清IgG的评价

Evaluation of up-converting phosphor-lateral flow method for rapid detection of IgG antibodies in echinococcosis patients

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