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Table of Content

    30 November 1987, Volume 5 Issue 4
    IDENTIFICATION OF PROTECTIVE ACTIVITY OF MONOCLONAL ANTIBODIES AGAINST PLASMODIUM FALCIPARUM
    1987, 5(4):  244-247. 
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    Seventeen monoclonal antibodies specific for P.falciparum were tested for their ability 10 inhibit the multiplication of a partially synchronized culture of P. falciparum by parasite counts. Seven of these antibodies, ranging from culture supernatant and ascitic fluid to purified IgG, were shown to have a dose-depsndent inhibitory effect on parasite growth in vitro. At a final concentration of 0.6 mg/ml, the inhibitory capacity of purified IgG was above 90% over a 3-day culture period, greater than that of the relevant polyclonal antibodies. These monoclonal antibodies caused agglutination of free merozoites, multiple invasion of merozoites within a single erythrocyte, and/or degeneration of schi-zonts, suggesting that there may be several acting points of the inhibitory monoclonal antibodies. However, the mechanisms of action of the inhibitory antibodies varied with different monoclonal antibodies.
    A STUDY ON MONOCLONAL ANTIBODY ELISA TEST TO DETECT MALARIA PARASITE ANTIGEN
    1987, 5(4):  248-250. 
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    This paper reports an inhibitory ELISA using monoclonal antibodies M26-32 to detect malaria parasite antigen. The characteristic of this method is to coat the polysterene microculture wells with rabbit anti-mouse IgG serum instead of P. yoelii or P. falci-parum. Examination of 69 microscopically positive and 88 negative blood samples collec-ted on filter paper revealed that, taking inhibition late≥9% as positive standard, the coincidence rate for both microscopically positive and negative samples is above 90%. The inhibition rate is closely related to parasite density, the lowest detectable level being 2.6 parasites/104 erythrocytes.
    SCANNING ELECTRON MICROSCOPIC STUDY ON THE LATE STAGE OF SPOROGONY OF PLASMODIUM VIVAX
    1987, 5(4):  251-252. 
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    Seven batches of Anopheles dims were used to take blood meal successively from 4 P. vivax-infected patients living in Guizhou Province after "informed consent". Morphological studies with scanning electron microscope on various stages of sporogony on days 9, 11 and 12 post-infection were carried out. The surface features of oocysts were similar to those of other species of the genus Plasmodium. The inner surface of oocyst wall appeared rough and bore many spinal processes (Fig. 5). From the early stage of sporoblast, budded the short cone-shaped sporozoites buds which gradually developed into sporozoites (Figs. 6~9). The sporozoite appeared elongated, curved and invested with smooth plasma-lemma. The microspore was located about 1/3 of the body from the anterior end which was somewhat truncated obliquely (Fig. 10).
    THERAPEUTIC EFFECTS OF 13 COMBINATIONS OF ANTIMALARIALS AGAINST PLASMODIUM BERGHEI "NS" STRAIN
    1987, 5(4):  253-255. 
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    The experimental effects of 13 combinations of antimalarials against P. berghei "NS"strain were observed with Berenbaum's method (1978). The results showed that combinations of piperaquine + nitroquine in the ratios of 1:1, 1:1/3 and 1/3:1, hydroxy-piperaquine + nitroquine (1:1,1:1/3, and 1/3:1) and pyronaridine + nitroquine (1:1 and 1/3:1) were synergistic.The combinations of piperaquine + pyronaridine (1:1) was additive while piperaquine + mefloquine (1:1) exhibited antagonism (Table 2).
    SUPPRESSIVE EFFECT OF 3 COMBINATIONS OF ANTIMALARIALS AGAINST PLASMODIVM CYNOMOLGI
    1987, 5(4):  256-258. 
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    Suppressive effect of piperaquine + nitroquine, hydroxypiperaquine + nitroquine and pyronaridine + nitroquine combinations in rhesus monkeys infected with Plasmo-dium cynomolgi were observed. The results suggested that piperaquine 3.5 mg/kg·d×7 + nitroquine 0.1 mg/kg·d×7, hydroxypiperaquine 3.5 mg/kg·d×7 + nitroquine 0.1 mg/kg·d×7 and pyronaridine 5 mg/kg·d×7 + nitroquine 0.1 mg/kg·d×7 can all cure the monkeys infected with P.cynomolgi. Until D105 after administration, recrudescence of parasitaemia did not occur in any of the test monkeys. By contrast, after piperaquine 3.5 mg/kg·d×7,hydroxypiperaquine 3.5 mg/kg·d×7, pyronaridine 5mg/kg·d×7 or 10mg/kg·d ×7 and nitroquine ×0.1 mg/kg·d×7 or 0.15×7 were administratered respectively, para-sitaemia in all of the test monkeys reappeared within one month.
    SURVEY OF HUMAN PARASITES IN FANCHANG COUNTY, ANHUI PROVINCE
    1987, 5(4):  259-261. 
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    It is in urgent need to understand the distribution and prevalence of parasitic infections of humans for the planning of a national program for disease control, A pilot study was made in Fanchang county, Anhui province, to get necessary experience.Six pilot sites were selected in the county according to different topography and socio-economic situation. Four methods, i. e. iodine direct smear, Kato-Katz thick smear, adhesive-tape technic and fecal culture were used. For Paragonimus infection, intradermal test was carried out, to be followed by sputum examination of cases with positive skin test.Of 3 127 persons from the sites examined, the overall parasitic infection rate was 90.6%. The percentages of cases infected by 1, 2, 3, 4, 5 or 6 kinds of parasites in the population were 24.5%, 29.7%, 20.4%, 3.9%, 0.5% and 0.03% respectively.The following species of protozoa, nematode, trematode and cestode were found in the population: Entameba histolytica, E. coli, Giardia lamblia; Ascaris lumbricoides, Tri-churis trichiura, Ancylostoma duodenale, Necator americanus, Enterobius vermicularis, Strongyloides stercoralis, Trichostrongylus sp.; Schistosoma japonicum, Fasciolopsis bus-ki, Paragonimus -westermani, Clonorchis sinensis, Heterophyid flukes, Echinostomatid. flukes and Hymenolepis diminuta. Among them, Giardia lamblia, Trichostrongylus sp. Heterophyid flukes and Echinostomatid flukes were reported for the first time in Anhui province.The pilot survey reveals that there are 17 species of parasites infecting humans and the prevalence of some infections is quite high in Fanchang, where the socio-economical development ranks medium in the country.
    EXPERIMENTAL THERAPEUTIC EFFICACY OF A NEW ANTI-HOOKWORM DRUG--TRIBENDIMIDIN
    1987, 5(4):  262-264. 
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    Tribendimidin, an analogue of amidantel,was synthesized by this institute. Its antheU mintic effects on rats infected with Nippostrongylus braziliensis, dogs infected with An-cylostoma caninum and hamsters infected with Necator americanus were evaluated in comparison with mebendazole, pyrantel pamoate and amidantel.When rats infected with Nippostrongylus braziliensis and dogs infected with An-cylostoma caninum were treated with tribendimidin at a single oral dose of 25mg/kg, the worm reduction rates were 99.3 and 91.9%, respectively. At the same dosage all Toxo-cara canis in dogs and a part of Syphacia mesocriceti in hamsters were expelled. The oral dosage of tribendimidin needed to cure hamsters infected with Necator americanus was 150 mg/kg.It was found that tribendimidin had the advantages of higher efficacy, lower dosage and shorter course of treatment. All parasites were expelled quickly after treatment. No indication was found of neither mutagenic nor teratogenic effect of tribendimidin.
    DETECTION OF ENZYME DISSOCIATED SCIC FROM SERA OFSCHISTOSOMIASIS JAPONICA BY TWO-DIRECTION COUNTER.IMMUNOELECTROPHORESIS
    1987, 5(4):  265-268. 
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    A new method of two-direction counter-immunoelectrophoresis (TD-CIEP and TD-ELACIEP) for detection of specific antigen and antibody of enzyme-dissociated schisto-some circulating immune complexes (SCIC) in infected rabbits and advanced schistosomia-sis cases was reported. Results showed that the detection rate was markedly increased in the enzyme-dissociated sera as compared with that of non-dissociated sera. The levels of antigen and antibodies were found to be correlated to the period and intensity of infection. The detected antigen was solely originated from the adult worm. Comparison of sensitivity of both methods showed that TD-ELACIEP was much more sensitive than TD-CIEP either for detection of antigen or antibodies. Of 54 sera of advanced schistosomiasis cases the detection rate of schistosome antigen or antibodies by TD-ELACIEP in enzyme-dissociated and non-dissociated serum samples was 50 and 25.9%, respectively. Only 9 cases( 16.7%) were COP positive with COP rate^5%. Sera from 25 healthy persons revealed no false positive reaction by either method. The present results indicated that TD-ELACIEP of dissociated SCIC may be helpful in immunological diagnosis of advanced schis-tasomiasis.
    CHANGES IN SERUM ANTIBODY LEVELS IN PATIENTS WITH SCHISTOSOMIASIS JAPONICA BEFORE AND AFTER TREATMENT WITH PYQUITON AND ITS SIGNIFICANCE
    1987, 5(4):  269-272. 
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    The Enzyme-linked Intraoval Precipitin test(ELIOP) and Indirect Hemagglutination test with a gut-associated antigen (GAA-IHA) were used to observe the kinetics of antibody levels in patients with schistosomiasis japonica before and after treatment with pyr quiton in two different endemic villages. The results showed that the antibody geometric mean reciprocal titer(GMRT) and positive rate decreased significantly in ova-negative patients 3-4 months after treatment; the decrease of GMRT as well as the positive rate of ELIOP in the village where snails had been eradicated were more markedly and earlier than those in the village where snails had not been eradicated, and the antibody level in young age groups tended to decrease more rapidly than that in older age groups. These suggest that the measurement of the kinetics of antibody levels in patients with schistosomiasis before and after treatment, especially in young age group, could be used to estimate the effectiveness of a control program soon after its performance and may be of value for surveilance of transmission in a given endemic area. This paper also noticed that the antibody levels after treatment detected by ELIOP decreased more rapidly than those by GAA-IHA, suggesting that ELIOP may be more promising in seroepidemiologica survey of schistosomiasis.
    OBSERVATION ON THE STABILITY OF SCHISTOSOME EGG OUTPUT IN FINAL HOST FAECES
    1987, 5(4):  273-277. 
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    Faecal egg count was made in 80 schistosomiasis japomca patients with Kato-Katz quantitative smear method every two days, and daily output of eggs from feces were counted in 7 schistosome-infected dogs with the same technique.Variance analysis method was performed of log-transformed data. The results showed that there was statistically significant difference among the geometric means of EPG and EPD during 3 months in the same host.The egg output of adult S. japonicum in the feces was unstable in a certain period of time, but EPG and EPD appeared to fluctuate rhythmically every 4-6 days. For epi-demiological survey as well as for evaluation of the control effect, it is suggested that when faecal examinations are made with four smears every two days for 3-4 consecutive times, the average egg output may objectively reflect the intensity of infection.
    PRELIMINARY OBSERVATION ON SOME ASPECTS OFBIOLOGY AND PROTEIN BANDS OF METACERCARIAE OFEUPARAGONIMUS CENOCOPIOSUS
    1987, 5(4):  278-280. 
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    Some biological and biochemical characteristics of metacercaria of Euparagonimus ce-nocopiosus obtained from crabs in the mountainous area of south Anhui, were compared with those of Paragonimus westermani from the same batch of crabs.The metacercariae of E. cenocopiosus prefer the location in heart rather than in the muscle of crabs. The average infection rate of crabs was 42.8% by E. cenocopiosus, while that by P. westermani was 100%. The infection rate of rats with E. cenocopiosus was 3/6 and 10/10 with P. westermani and the recovery rate of worm of these two species was 4.5-8.8% and 22-72.2% respectively. The number and pattern of protein bands of metacercaria of these two species by disc-PAGE and IEF technics were different.
    ELECTRON MICROSCOPIC STUDIES ON THE EFFECT OFCORYDALIS STRICT A STEPH ON HUMAN ECHINOCOCCUSGRANULOSUS AND PROTOSCOLICES
    1987, 5(4):  281-283. 
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    The ultrastructural changes of the germinal membrane of human Echinococcus granu-losus cyst and protoscolices treated with total alkaloids isolated from Corydalis stricta Steph for 30 days(15mg/kg·d, po) were observed. Cysts from the total alkaloid-treated patients were shown by transmission electron microscope to have disturbance in the arrangement of organelles dilatation and disruption, of microtubules, increase in size and number of lysosomes, decrease in number of Golgi bodies, and degeneration of mitochondria. Under scanning electron microscope many pits were observed arising from the outer and inner surfaces of the germinal membrane together with disruption of the external plasma membrane, hook detachment, sucker deformity of evaginated protoscolices. It seems clear that the total alkaloid has a profound intracellular effect on Echinococcus granulosus cysts and protoscolices in humans and might be a promising drug for human hydatid disease.
    OBSERVATION ON ULTRASTRUCTURE OF THE CYSTS ANDTHE TEGUMENT SURFACE OF PROTOSCOLICES OF ECHINOCOCCUS GRANVLOSUS FROM HUMAN CASES
    1987, 5(4):  284-287. 
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    The fine structures of 10 cysts and the tegument surface of protoscolices of E. gra-nulosus from human lungs and livers were observed by means of transmission(TEM) and scanning electron microscopy(SEM). Examined by TEM, the laminated layer is made up of microfibrillate matrix with aggregates of electron-dense materials (Fig. 1). The germinal layer contains microtriches, tegument and tegumental cells, etc. Larger vesicles found near the inner margin of the cysts (Fig. 3)are presumably young brood capsules. Irregular electron-dense granules adhere to the vesicles and the inner membrane. The structure is similar to myelin sheath which lies in germinal layers (Fig. 4). By using SEM, most of the protoscolices appear invaginated. They are characterized by a smooth contour without microtriches. No orifice is visualized at the anterior end where the apical portion is invaginated (Fig. 6, 7). The posterior end is pedundle-like.Generally, the tegument surface of the eyaginated protoscolices could be divided into four regions: the rostellum region with hooks and long, slender, filamentous microtriches; the mid portion containing four suckers and blade-like microtriches; in the rear part, tegumental indentations forming longitudinal, transverse and irregular furrows, the tegument surface bearing many small cylindrical projections which might be the undeveloped microtriches; the peduncle region resembling that of invaginated protoscolices (Figs. 8-10).
    ANTHELMINTICS AGAINST MIGRATING STAGE LARVAE OF ANCYLOSTOMA DVODENALE IN MICE
    1987, 5(4):  288-290. 
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    Mice infected in our laboratory with Ancylostoma duodenale were used to evaluate the effect of levamisole, albendazole, pyquiton, mebendazole and amidantel against the migrating stage larvae. The results showed that levamisole 34 mg/kg·d×3 and albendazole 181.4mg/kg·d×5 exerted promising effect and the reduction rates of larvae were as high as 97.3% and 97.5% respectively.It is recommended that levamisole and albendazole could be used in field trials to control both adults and migrating larvae of A. duodenale.
    STUDIES ON THE REAPPEARANCE OF GAMETOCYTES OF PLASMODIVM BERGBEI
    1987, 5(4):  291-293. 
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    The Plasmodium berghei parent line which had lost the ability of producing gameto-cytes was maintained soly by syringe passage for more than 50 years. After transmitting the parent line parasites under pyronaridine pressure for 114-134 syringe passages, a pyronaridine-resistant(RP) line at a high level 0300-fold) emerged. When the RP line Passage 114 and Passage 134 were further transmitted through 15-21 syringe passages in the absence of drug pressure, gametocytes abruptly reappeared in their blood. These reappeared gametocytes were fertile and the sporozoites developed in A. stephensi were infective to mice. The P. berghei RP line parasites through cyclic transmission retained their resistance to pyronaridine at a high level and cross-resisted highly to chloroquine.
    STUDIES ON CRITERIA OF TRANSMISSION-INTERRUPTION OF FILARIASIS DUE TO BRUGIA MALAYI
    1987, 5(4):  294-296. 
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    A field investigation of critical transmission level of malayan filariasis was carried out in a low endemic area from 1982 to 1984 in Mojiaqiao village of Yuhang County, Zhejiang Province, where Anopheles hyrcanus sinensis acts as vector of the disease. Based on the findings of three consecutive years, we are convinced that a microfilarial density below 5/60μl peripheral blood is no longer significant in the spreading of the disease. And, if the microfilariemia rate is reduced to less than 1% and the microfilarial density is as low as 5/60μl or less, the remaining cases with microfilariemia would gradually be self-cured and the transmission of the disease would virtually be interrupted. Therefore, finding out and treating those subjects with high microfilarial density as soon as possible are key measures to prevent the spreading of filariasis malayi in low endemic area. In addition, there is no longer any need for repeated surveys and treatments. The necessary work is to strengthen monitoring activities. As a result, much manpower and material resources could be saved.