Table of Content

30 April 2007, Volume 25 Issue 2

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论著

Isolation and Identification of an Isolate of Cow-origin Cryptosporidium sp.

LIUHai-peng;CAOJian-ping;SHENYu-juan;CHENYou-gui;LIXiao-hong;LUWei-yuan;XUYu-xin;LIUYi-sheng-LIUShu-xian;ZHOUXiao-nong;TANGLin-hua

2007, 25(2):  1-86. 

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【Abstract】 Objective To isolate and identify Cryptosporidium oocysts from feces of naturally infected cow. Methods Fecal samples were collected from Cryptosporidium infected cows confirmed by modified acid-fast staining method. Oocysts were isolated and purified with Sheather sucrose density gradient centrifugation technique. Genomic DNA was isolated with Chelex-100. Both primers were designed to amplify Cryptosporidium small subunit ribosome RNA gene （SSU rRNA） and Cryptosporidium oocyst wall protein gene （COWP）， respectively. The PCR products were cloned into pGEM-T and pGEM-T Easy vector and sequenced subsequently. Homology and phylogeny were analyzed with BLASTn and MEGA software. Results The results suggested that the size of oocysts was （7.4±0.32） μm by（5.4±0.21） μm and the ratio of length and width was 1.37±0.07（n=20）. BLASTn revealed that the identity of SSU rRNA and COWP gene of Cryptosporidium isolated from cow to the counterparts of C.andersoni was 100% and 99% respectively. Phylogenetic reconstruction placed the isolated Cryptosporidium within the C.andersoni clade based on the sequence of SSU rRNA and COWP gene. Conclusion What isolated from naturally infected cow feces has been identified as C. andersoni.

Isolation and Purification of Antibacterial Peptides from the Larvae Secretion of Housefly and the Characteristics

GUOGuo;WUJian-wei;FUPing;QINRong-gui;ZHANGYong;SONGZhi-kui

2007, 25(2):  2-92. 

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【Abstract】 Objective To isolate and purify the antibacterial peptides from larvae secretion of housefly （Musca domestica） and study their partial characteristics. Methods Protein isolation and purification were performed by routine process， namely， ultrafiltration， solid phase extraction （SPE） and reversed-phase high-performance liquid chroma-tography （RP-HPLC）. The minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of the antibacterial peptides were examined. The antibacterial effect of peptides was studied in nutritive medium with different pH value（pH 5.0－10.0）， divalent cations （Mg2+： （0.5×10^-3－10.0×10^-3）mol/L， Na⁺、 K⁺：（10×10^-3～100×10^-3）mol/L）， and serum content（12.5％－75％）. Results Molecular weight of the peptides was about Mr 3 000－30 000 after ultrafiltration. The fractions eluted with 20%， 30%， 70％， and 80％ of acetonitrile （ACN） all showed antibacterial activity by solid phase extraction. The fractions eluted with 70% ACN showed strongest and stablest antibacterial activity which was further purified by RP-HPLC. Two sub-fractions appeared at around RT 15.5 min and 18.5 min were obtained with antibacterial activity. The MIC to those standard Escherichia coli， Pseudomonas aeruginosa， Staphylococcus aureus， and Bacillus subtilis was 32.738 0， 16.368 8， 65.475 0 and 32.738 0 μg/ml respectively. In the nutritive medium of pH 6.0－9.0， different divalent cations and serum content， the increment of A₅₇₀ in experiment groups was less than 0.05， while that of the control group was greater than 0.3 （P<0.01）. Conclusion SPE and RP-HPLC have been effective in purifying the antibacterial peptides which show strong activity in different conditions.

In vitro Cultivation of Echinococcus multilocularis Metacestodes and Observation of Their Growth

ZHANGYa-lou;WANGTing-ting;ZHOUXiao-tao;JIANGTao;QIXin-wei;LIUHui;LIUTao;LINRen-yong;WENHao

2007, 25(2):  3-96. 

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【Abstract】 Objective To observe the growth and development of Echinococcus multilocularis metacestodes under in vitro cultivation. Methods Hepatoma cell line was used for the cultivation. The number and morphology of the cysts were observed under light microscope. The parasite tissue was fixed and observed under electron microscope. Results During the first 21 days of cultivation, metacestodes in cyst-suspension derived cultures increased dramatically, and from the 22nd day on, the number of cysts remained as 6－7 times more than that of the 3rd－4th day of culture. Budding of new cysts was observed and the diameter of the cysts increased as time went on. On the 22nd day, larger cysts occupied 30%. Cysts were found with morphology between protoscolex and metacestode.　 Conclusion 　An in vitro cultivation for the cysts of E. multilocularis has been established and basic feature of growth and development of the larvae observed.

Preparation of Monoclonal Antibodies Against the Adhesion Protein 33 of Trichomonas vaginalis

HUANGHui-cong;YUShi-fang;CAIMing;TANFeng;ZHENGXiao-yun;PANChang-wang

2007, 25(2):  4-100. 

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Objective To prepare and characterize the monoclonal antibodies （McAbs） against recombinant adhesion protein 33 （AP33） of Trichomonas vaginalis. Methods The purified recombinant fusion protein AP33 was used as antigen to immunize BALB/c mice. Sp2/0 myeloma cells were fused with the splenocytes from immunized BALB/c mice. After ELISA screening and 4 times of limited dilution， 5 positive hybridoma cell lines were obtained， and the biological properties of the McAbs were identified by Western blotting. Indirect immunofluorescent antibody test （IFAT） was performed and the inhibition effect of McAbs on the cytoadherence of T.richomonas vaginalis to HeLa cell was assayed. Results Western blotting demonstrated that 5 McAbs， designated as 4A2， 4F11， 4F8， 4E7 and 4H11， specifically combined with the recombinant AP33 of T.vaginalis. The McAbs were IgG1 isotypes. Four of them （4F11， 4F8， 4E7 and 4H11） showed parasite recognition by IFAT. Parasite cytoadherence to a monolayer of HeLa cells was inhibited in vitro with a inhibition rate of 50.08%， 65.03%， 50.70% and 49.08% by the 4 McAbs under a concentration of 200， 200， 400 and 200 μg/ml， respectively. Conclusions The prepared McAbs against the recombinant AP33 show a protective inhibition on cytoadherence of Trichomonas vaginalis in vitro.

Effect of Different Adjuvant Formulations on the Induced Protection of Mice Immunized With Recombinant Protein Ts87 of Trichinella spiralis

LIQiang;YANGJing;YANGYa-ping;GUYuan;LIUZhi-ying;HUANGSong;ZHUXin-ping

2007, 25(2):  5-105. 

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Objective To study the effect of 6 formulations of adjuvants on immunoprotection in mice induced by the recombinant Ts87 protein （rTs87） of Trichinella spiralis. Methods ICR mice were vaccinated subcutaneously 3 times in 2-week intervals with rTs87 and different adjuvants CFA or IFA， Montanide IMS1312， Montanide ISA720， Quil-A and Al(OH)3， respectively. The antibody response was detected by ELISA and the purified rTs87 was analyzed by SDS-PAGE and Western blot. The protection induced in mice was then evaluated by muscle larvae reduction after Trichinella spiralis challenge. Results The results clearly showed that all the 6 adjuvants were able to enhance specific anti-rTs87 IgG response. SDS-PAGE and Western blot revealed that the molecular weight of expressed protein was around Mr 40 000 and could be recognized by sera from mice infected with Trichinella spiralis and mice immunized with rTs87. The reduction rate of muscle larvae was 49.4%， 49.2%， 63.5%， 65.1% and 70.8%， respectively. Conclusion Protective immunity of rTs87 can be enhanced by adjuvant CFA or IFA， IMS1312， ISA720， Quil-A and Al（OH）3， and better effect is observed with the latter 3 adjuvants.

Study on the Haplotypes of pfcrt Polymorphism from Hainan Province

WANGAn-ping;GAOQi;GUYa-ping;WANGShan-qing;WANGGuang-ze;LINShi-gan;MENGFeng

2007, 25(2):  6-108. 

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Objective To develop a method for comparing the haplotypes of pfcrt polymorphism of Hainan Province with those from other areas of the world. Methods Nested PCR was used to amplify the polymorphic region including codon 72 to 76 and 97 of pfcrt gene. The PCR products were digested by ApoI restriction endonuclease to determine the allelic types. According to the allelic types, 6 products from each of mutant type and wild type were sequenced to analyze the haplotypes of pfcrt polymorphism. Results Bands in size of 195 bp appeared in all 19 samples. After ApoI digestion, 11 samples contained one ApoI site when codon 76 of the pfcrt gene codes for lysine（K76）, which were visualized by the presence of 98 bp and 97 bp restriction fragments. The DNA sequencing revealed that 6 samples of cloroquine resistant P. falciparum carried pfcrt alleles encoding an amino acid haplotype of CVIET （residues 72-76）, and the haplotype of CVMNK was found in other 6 samples with wild type pfcrt gene. Conclusion Haplotypes of pfcrt polymorphism from Hainan were the same as those from southeast Asia and Africa.

Change of Splenocyte Lymphokines in Mice Induced by Recombinant BCG-Eg95 Vaccine against Echinococcus granulosus

LIWen-gui;WANGHong;ZHUYou-ming

2007, 25(2):  7-113. 

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Objective To investigate the reduction of hydatid cyst weight and change of splenocyte lymphokines in mice immunized with recombinant BCG-Eg95 vaccine of Echinococcus granulosus（Eg）. Methods BALB/C mice were subcutaneously, intranasally, orally and intramuscularly vaccinated respectively, with BCG and PBS served as controls. The mice were challenged with Eg protoscolices 8 weeks after vaccination and sacrificed in 18 weeks after infection. The weight of hydatid cyst was measured and reduction of the weight was obtained, spleens were used to separate splenocytes which were cultured under stimulation with EgAg or ConA. The supernatant was collected to measure the level of IL-2、 IFN-γ、TNF-α and IL-4 by ELISA. Results The hydatid cyst weight reduced by 45.77%, 18.20%, 88.05% and 92.46% respectively in the 4 immunization groups. In comparison with PBS control， the level of IL-2, IFN-γ and TNF-α was （30.0±0） pg/ml, （65.0±0） pg/ml and （425.0±10.7） pg/ml respectively in the intramuscular group with a significant increase, but that of IL-4 decreased， with a value of （10.0±0） pg/ml. Conclusion The recombinant BCG-Eg95 vaccination induces Th1 response in mice against challenge infection.

Evaluation on Schistosomiasis Control Effect of the Intervention Measures Adapted to the Ecological Environment Changes in Jiang Han Plain Due to Establishment of the Three Gorges Dam

WANGWen-liang;ZHANGHan-zhong;CAIZong-da;QINQin;LIUFeng-chun;XUXing-jian;WEIFeng-hua;ZHENGJiang

2007, 25(2):  8-119. 

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Objective To evaluate the effect of the intervention measures for schistosomiasis control adapted to the ecological environment changes in Jiang Han plain caused by the establishment of the Three Gorges Dam. Methods Four villages in Qianjiang City were selected to implement paddy-upland rotation, crawfish-paddy alternation, water control and soil improvement, and adjusting agricultural structure to rebuild the waterlogging low yielding land and to change the snail habitat environment respectively. The snail habitat area, mean density of living snails and prevalence of schistosomi-asis in human and cattle were compared with those of the control villages. Miracidia hatching methods were used to examine the prevalence in human and cattle. Results In the four experimental villages, the snail-ridden area decreased by 100%, 51.35%, 62.16% and 87.88% respectively; mean density of living snails decreased by 100%, 69.41%, 52.30% and 75.77%, with a t value of 9.37, 4.91, 2.31 and 9.16, I′<0.01. Human prevalence of schistosomiasis in 2005 in village with crawfish-paddy alteration decreased significantly than control （x²=39.84，I′<0.01）; decreased by 73.10% in village with water control and soil improvement in 1990 than in 1987 （x²=236.10，P<0.01）. Conclusion Implementation of the four intervention measures reaches a remarkable benefit in reforming snail habitat and protecting environment, which can be recommended to the inner embankment type endemic regions.

实验研究

Expression of Allergen Bla g 2 from Blattella germanica in Pichia pastoris

LIUZhi-gang;HEYi-hua

2007, 25(2):  9-123. 

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Objective To express the major allergen of Blattella germanica （Bla g 2） in Pichia pastoris and obtain the soluble protein. Methods The known Bla g 2 gene was used to design the primers which had the restriction enzyme sites. PCR method was applied to obtain the Bla g 2 gene. The gene fragment was then cut and ligated with the Pichia expression vector pGAPZaA, resulting in a recombinant plasmid pGAPZaA-Bla g 2. The linearized pGAPZaA-Bla g 2 was transformed into Pichia pastoris GS115 through electroporation, then screened to positive transformants, and the protein was expressed in YPD medium. Purification of the recombinant protein was achieved by metal （Ni²⁺） chelating affinity chromatography and Western-blotting assay indicated its IgE binding capacity. Results With the expressed recombinant protein, SDS-PAGE showed the presence of the product in the supernatant of the culture with Mr 45 000. After 3 days culture, the recombinant protein occupied 50% of the total proteins in the supernatant. The recombinant protein was purified and Western-blot demonstrated an adequate IgE binding capacity of the product. Conclusion A recombinant protein of Bla g 2 has been obtained, which is soluble in the supernatant and therefore can avoid a process of denaturalization and renaturation of the recombinant.

Cloning of Amastin Gene of Leishmania donovani Isolates from Sichuan Province and its Expression in Eucaryotic System

LIJin-fu;CHENJian-ping;TIANYu;YANGZhi-wei;MAYing;HUXiao-su

2007, 25(2):  10-128. 

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Objective To clone and express the amastin gene of two Leishmania donovani isolates from Sichuan Province of China. Methods Amastin gene was amplified from nuclear DNA of two L.donovani isolates， cloned into pcDNA3.1（+）， and sequenced by the dideoxy chain termination. NIH3T3 cell was transfected by recombinant plasmid. Transient expression of amastin gene was detected by immunofluoresence and stable expression was detected by RT-PCR and Western blot. Results Amastin gene of both isolates was 552 bp. Sequence analysis showed that the similarity was 86% between the two isolates. A high green fluorescence was found on the cell membrane and inside the cell. The NIH3T3 cell was transfected by the recombinant plasmid successfully. Amastin gene was obtained by RT-PCR from the transfected NIH3T3 cells. Western blot analysis showed that there was a protein about Mr 20 000 in lysate of the transfected NIH3T3 cells， indicating that the amastin gene was expressed in the cells. Conclusion Amastin gene of the two L.donovani isolates has been cloned and the gene can be expressed stably in the NIH3T3 cell.

Continuous Axenic Cultivation of Pneumocystis carinii Isolated from the Bronchoalveolar Lavage Fluid of Infected Rat

HUANGMin-jun;ANYi-jun;LIShu-zhen;LUSi-qi;GUOZeng-zhu

2007, 25(2):  11-132. 

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Objective To establish axenic cultivation of Pneumocystis carinii （P.c）. Methods The organisms of P.c were isolated from the bronchoalveolar lavage fluid （BALF） of the rats with Pneumocystis carinii pneumonia （PCP） and cultured in a medium which was based on IMDM（GIBCO） supplemented with S-adenosyl-L-methionine, putrescine, N-acetyl glucosamine, putrescine, L-cysteine and L-glutamine, and newborn calf serum. The organisms cultured in the system were identified by observing the morphology of cysts in smears stained with Gomori′s methenamine silver nitrate stain （GMS）. Ultrastructure of the cysts/trophozoites was examined by transmission electron microscopy. The sequences of mitochondrial large ribosomal DNA subunit of the cutured organisms were compared with the Pneumocysti carinii f.sp.ratti variant isolate （GenBank No U20173） and Pneumocystis carinii f.sp.hominis （GenBank No M58605）. Results Five isolates of P.carinii received from BALF of 8 rats with PCP were cultured axenically and continuously in the system. The cultured organisms could be stored in frozen condition and used to reinitiate culture, and were amplified by 19-22 times within 72 h. The morphology, ultrastructure and gene sequencing of the cultured organisms confirmed that the isolated organisms were P.carinii. Conclusion Five continuously and axenicly cultured isolates of P.carinii have been received.

Anti-mite Activity and Skin Safety of Herba taraxaci Extract for Demodex folliculorum

TIANYe;LIChao-pin;DENGYun

2007, 25(2):  12-136. 

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Objective To test the in vitro effect of the extract of Herba taraxaci on Demodex folliculorum. Methods Active Demodex folliculorum were obtained from patients with moderate to severe demodex infestation. Herba taraxaci and Radix stemonae were extracted respectively with 80% ethanol under 85℃， and a preparation with a concentration of 200mg/ml was made. The extractions were used in vitro to examine the anti-mite activity by observing time of killing mites. Physiological saline and Radix stemonae extraction served as blank control and positive control respectively.PH value of Herba Taraxaci extract was noted. Skin irritation test of normal and wounded skin and acute toxicity test were carried out with rabbit shin, and Herba taraxaci and 75% ethanol were served as experiment and control medicine. Results Motion and morphology of the mites considerably changed with the effect of Herba taraxaci extract. The time of mite-killing was （1.50±0.65）min with Herba taraxaci and （3.53±1.04）min with Radix stemonae respectively （P＜0.01） and over 120 min with physiological saline. pH value of Herba taraxaci extract was 5.00±0.28. Score for irritation to normal and wounded rabbit skin was 0 and 0.3 respectively， and acute toxicity test showed no significant toxicity. Conclusion Herba taraxaci extract shows a remarkable in vitro activity to Demodex folliculorum with skin safety.

Effect of N-acetylcysteine on the Egg Granuloma in Hepatic Tissue of Mice with Schistosomasis japonica

FANZhi-gang;ZHANGLing-min;LIKai-jie;LIWei;ZHUPei-xan;YANGGuang

2007, 25(2):  13-140. 

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Objective To study the effect of N-acetylcysteine （NAC） on the egg granuloma in hepatic tissue of mice infected with Schistosoma japonicum. Methods 36 mice were randomly divided into normal group, infected group and NAC group, each with 12 mice. The mice in the latter two groups were each infected with 25±2 cercariae of S.japonicum through the skin of abdomen. NAC solution was orally given to the mice of NAC group, 200 mg/kg, 2 times/d from the day of infection through to the 42nd day. Mice in the other 2 groups were given 2 ml normal saline daily. The mice were all sacrificed at the end of the 42nd day and their livers were collected for pathologic observation. Area of the egg granuloma was measured with computer image analysis software. Concentration of nitric oxide （NO） and reduced glutathione hormone （GSH）, and the activity of glutathione peroxidase （GSH-PX） in serum and hepatic tissue, and the activity of inducible nitric oxide synthase （iNOS） in the hepatic tissue were all detected. Results Number of the single egg granuloma of “+、++、+++” grade were 1.80±0.25、 1.37±0.23 and 0.53±0.15 respectively in NAC treated group, which were less than those of infected group （3.70±0.28、 2.77±0.25 and 2.00±0.14 respectively） （P＜0.05）. The serum NO and GSH concentration was 0.53±0.17 and 229.66±9.47 respectively in NAC group, lower than those of infected group （2.64±0.31 and 312.47±18.55 respectively） （P＜0.05）, but its GSH-PX activity was 1101.99±140.81, higher than that of infected group （663.66±25.59） （P＜0.05）. The concentration of NO and GSH, and the activity of iNOS and GSH-PX in hepatic tissue of NAC group were 6.85±0.30、 13.44±0.40、 358.40±19.15 and 110.84±10.93 respectively, lower than those in infected group （8.26±1.69、 28.40±0.56、 1132.44±52.82 and 226.26±16.25 respectively） （P＜0.05）. Conclusion NAC may have the effect of retarding pathological change of the liver, which may associate with the decrease of NO and GSH in serum and hepatic tissue and iNOS activity in the tissue.

Studies on Haemocytes of Oncomelania hupensis

ZHANGHong-mei;ZHUGEHong-xiang;WANGYu-fang;Gongwei;

2007, 25(2):  14-145. 

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Objectives To establish method for collecting haemocytes of Oncomelania hupensis and study its morphology and immunological importance. Methods Referring to the method of haemocytes collection from peripheral lymphoid organ, suspension technique was used for collection of haemocytes from snails, which were then Giemsa-stained and observed under microscope. Stained by gentian violet, number of haemocytes was counted and compared with that of conventional squashing method and needling method by ANOVA and Dunnett-t test. Supernatant from freeze thawing hae-mocytes was applied for the tests of immuno-precipitation, bacteriostasis, and phagocytosis. SDS-PAGE was used to an-alyze relative molecular mass of protein ingredients. Results Four kinds of haemocytes were found: round cells with filiform filopodia, acidophilic and basophilic round cells both without filiform filopodia, and spindle cells. The average diameter of the 4 type cells was 10.93, 6.13, 6.08, and 11.06 μm, and occupied 50%, 30%, 5%, and 15% respectively. The mean of haemocytes received from suspension, squashing and needling methods was 15 000, 6 600 and 300/ml respectively. ANOVA analysis showed F=281.47, P＜0.01, and Dunnett-t test revealed t₁=15.67, P＜0.01 between suspension and squashing methods, and t₂=24.50, P＜0.01 between suspension and needling method. The supernatant of haemocytes showed precipitation with SEA, bacteriostasis with Staphylococcus aureus and Escherichia coli, and 86% phagocytosis and 46% bactericide to Candida albicans. SDS-PAGE revealed approximate Mr 112 300, 107 100, 972 00, 73 500, 600 00, 12 000 of the protein ingredients of the haemocytes. Conclusions Large amount of haemocytes has been obtained from the snails by suspension method, and the cells show immunological activities.

现场研究

Observation on the Change of Anti-S.japonicum Antibody Level inPopulation Migrated from Outside Embankment to New Town

WENLi-yong;LUShao-hong;CHENJun-hu;ZHANGJian-feng;YULi-ling;DINGJian-zu;YANXiao-lan;SHENLi-ying;ZHENGWei;GAOLu-lu;WANGTian-ping;ZHANGShi-qing;CHENGeng-xin;YEYun;ZHOUXiao-nong;ZHENGJiang

2007, 25(2):  15-149. 

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Objective To detect the change of the anti-S.japonicum antibody level after people migrated from outside embankment to newly established town. Methods Three pilot spots were established for the investigation: one spot that both inhabitancy and cultivation disused （A）, one spot that only inhabitancy disused but farming continued （B） and the third one served as control （C）. DIGFA and ELISA were used to detect the antibody level in the populations from 2002 to 2005. Results The positive rate of anti-S. japonicum antibody declined significantly from 6.63% to 3.52% by DIGFA and from 7.26% to 3.71% by ELISA at spot A （χ²=5.2625， P<0.05； χ²=6.3296， P<0.05, respectively）. There was no significant difference on the positive rate of antibody in spots B and C. The average A₄₅₀ value of ELISA in the three spots was statistically analyzed by One-Way ANOVA. It was only in spot B that the average A₄₅₀ value declined from 0.182 in 2003 to 0.147 in 2005 （P<0.01）. Conclusion The anti-S.japonicum antibody level in human population has decreased at certain degree after they migrated from outside embankment to new town.

综述

Progress on the Technologies of Prediction on Malaria and Mosquito Vectors

ZHUJi-min;TANGLin-hua

2007, 25(2):  16-153. 

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Various factors can impact the endemicity of malaria, proper monitoring and forecasting of the disease is of great significance. This article reviews the ways of malaria prediction including the newly developed technology of GIS/RS.

Update on Host Cell Invasion by Toxoplasma gondii

LIXue-rui;WANGYan-hua;ZHAOXiang-zhong;ZHANGDe-lin

2007, 25(2):  17-157. 

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This paper reviewed the importance of micronemes, dense granules, rhoptry and major surface proteins of Toxoplasma gondii, and of calcium during the host cell invasion as well as the role of T. gondii proteases in the folding and processing of these proteins.

研究简报

Infection Status of Common Intestinal Soil-borne Nematodes in Childrenfrom 3 to 6 Years Old in Kindergartens of Hangzhou

YEHuan;ZHANGZhi-liang;LUODong-jiao;ZHANGRen;YANGJun;HEGui-kuen

2007, 25(2):  18-159. 

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1 667 children of 3 to 6 years old were inspected randomly in kindergartens of Hangzhou from April to June 2006. Enterobius vermicularis eggs were detected by cellophane swab technique. Eggs of Ascaris lumbricoides， Trichuris trichiura and hookworms in fresh stool samples were examined by Kato-Katz thick smear and staturated brine floatation. 216 children were found to have infected with intestinal nematodes （12.96%）. The prevalence of E.vermicularis， A.lumbricoides，T.trichiura and hookworm was 4.44%， 8.28%， 0.54% and 0.24% respectively. Higher prevalence has been found in kindergartens with poorer environment and sanitation.

Preliminary Test of Phlebotomus Surveillance by Light-traps

GUDeng-an;JINChang-fa;LANQin-xian;ZHANGChou-ji;LIFan;ZHANGYi

2007, 25(2):  19-封三. 

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Different light-traps were tested in Wenxian of Gansu Province， including the light-traps without lamp and with glucose solution as attractant. Results showed that the light-traps attracted more mosquitoes and other insects than sandflies， and it became difficult to pick up the sandflies from gathering packet; the light-trap without lamp captured smaller amount of sandflies but much less other insects; glucose showed no significant effect in attracting sandflies. The existing light-traps are not so effective for Phlebotomus surveillance， and it is suggested that more effective attractants be tried.