华支睾吸虫成虫体被结构扫描电镜和透射电镜观察

中国寄生虫学与寄生虫病杂志 ›› 2018, Vol. 36 ›› Issue (2): 184-186.

华支睾吸虫成虫体被结构扫描电镜和透射电镜观察

石云良¹, 万孝玲¹, 蒋智华¹, 成晓静², 杨益超^1,^*()

1 广西壮族自治区疾病预防控制中心寄生虫病预防控制所,南宁530028
2 生命科学研究院电镜室,广西医科大学生命科学研究院,南宁530021

收稿日期:2017-06-08 出版日期:2018-04-28 发布日期:2018-04-24
通讯作者: 杨益超
基金资助:
广西壮族自治区疾病预防控制中心青年基金（No. 201501）;广西自然科学基金(No. 2012GXNSFAA276033)

Scanning electron microscopic and transmission electron microscopic observations of the tegument structure of adult Clonorchis sinensis

Yun-liang SHI¹, Xiao-ling WAN¹, Zhi-hua JIANG¹, Xiao-jing CHENG², Yi-chao YANG^1,^*()

1 Institute of Parasitic Disease Control and Prevention, Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China
2 Electron Microscopy Laboratory of Life Science Institute, Guangxi Medical University, Nanning 530021, China

Received:2017-06-08 Online:2018-04-28 Published:2018-04-24
Contact: Yi-chao YANG
Supported by:
Supported by Young Foundation of Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention（No. 201501）and Guangxi Provincial Natural Science Foundation (No. 2012GXNSFAA276033)

摘要/Abstract

摘要：

为了解华支睾吸虫体被的超微结构特征,用华支睾吸虫囊蚴实验感染大鼠（100个囊蚴/鼠）,感染后40 d,收集华支睾吸虫成虫,应用扫描电镜技术观察虫体体表,应用透射电镜观察虫体体被结构。扫描电镜下可见,虫体体表有许多结节突起,突起之间有丝状物连接;虫体的感觉乳突突出于表面,大小和分布不均。透射电镜下可见,虫体的表皮由体被、肌层、细胞体层组成。体被表面覆盖许多突起,突起内有线粒体、分泌颗粒和分泌小泡,最外层的突起有脱离虫体的趋势。基质层内有球形和杆状两型分泌颗粒、分泌小泡、线粒体。肌层分布有环形肌和纵行肌2种。细胞体层内细胞核清晰,细胞质内可见两型特征性的分泌颗粒和分泌小泡。细胞体层和体被通过胞质桥相连。

关键词: 华支睾吸虫, 体被, 扫描电镜, 透射电镜

Abstract:

To study the ultrastructural characteristics of Clonorchis sinensis, scanning electron microscopy (SEM) was performed to observe the body surface of adult C. sinensis obtained from an infected rat, and transmission electron microscopy (TEM) was used to observe the tegument structure. By SEM, we found a number of nodules connected by filaments on the body surface of the parasite, with unevenly-distributed different sizes of sense papillae protruding from the body surface. The TEM showed that the epidermis of the C. sinensis was composed of tegument, the muscle layer and the cell layer. The surface of the tegument was covered with numerous protrusions containing mitochondria, secretory granules and secretory vesicles. The outermost-layer protrusions showed a tendency to detach from the tegument. The matrix contained spherical and rod-shaped secretory granules, secretory vesicles and mitochondria. The muscular layer was distributed with circular and longitudinal muscles. In the cell body layer, cell nuclei were clearly seen and the cytoplasm contained two types of secretory bodies and secretory vesicles. The cell body layer and the tegument were connected by cytoplasmic bridges.

Key words: Clonorchis sinensis, Tegument, Scanning electron microscopy, Transmission electron microscopy

中图分类号:

R383.22

石云良, 万孝玲, 蒋智华, 成晓静, 杨益超. 华支睾吸虫成虫体被结构扫描电镜和透射电镜观察[J]. 中国寄生虫学与寄生虫病杂志, 2018, 36(2): 184-186.

Yun-liang SHI, Xiao-ling WAN, Zhi-hua JIANG, Xiao-jing CHENG, Yi-chao YANG. Scanning electron microscopic and transmission electron microscopic observations of the tegument structure of adult Clonorchis sinensis[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2018, 36(2): 184-186.

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