新疆新源县多房棘球蚴病病例特征及其病原基因多态性分析

doi:10.12140/j.issn.1000-7423.2022.02.008

中国寄生虫学与寄生虫病杂志 ›› 2022, Vol. 40 ›› Issue (2): 181-186.doi: 10.12140/j.issn.1000-7423.2022.02.008

新疆新源县多房棘球蚴病病例特征及其病原基因多态性分析

才仁¹(), 任远², 米荣升³, 郭刚¹, 齐文静², 张壮志⁴, 郭宝平¹^,^*()

1.新疆医科大学第一附属医院,临床医学研究院,乌鲁木齐 830011
2.新疆医科大学省部共建中亚高发病成因与防治国家重点实验室,乌鲁木齐 830011
3.中国农业科学院上海兽医研究所,农业部动物产品质量安全生物性危害因子风险评估实验室（上海）农业部动物寄生虫学重点实验室,上海 200241
4.新疆畜牧科学院兽医研究所（新疆医科大学第一附属医院临床医学研究院）,乌鲁木齐 830013

收稿日期:2021-08-12 修回日期:2021-10-01 出版日期:2022-04-30 发布日期:2022-04-07
通讯作者: 郭宝平
作者简介:才仁（1984-）,男,本科,助理实验师,从事寄生虫学研究。E-mail： 181132786@qq.com
基金资助:
新疆维吾尔自治区自然基金(2019D01C315)

Characteristics of alveolar echinococcosis cases and genetic polymorphism of the parasite from Xinyuan County, Xinjiang

CAI Ren¹(), REN Yuan², MI Rong-sheng³, GUO Gang¹, QI Wen-jing², ZHANG Zhuang-zhi⁴, GUO Bao-ping¹^,^*()

1. Institute of Clinical Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
2. State Key Laboratory of Pathogenesis, Prevention and Treatment of Central Asian High Incidence Diseases, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
3. Key Laboratory of Animal Parasitology of Ministry of Agriculture, Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
4. Veterinary Research Institute, Xinjiang Academy of Animal Sciences (Animal Clinical Medical Research Center of Veterinary Research Institute, Xinjiang), Urumqi 830000, China

Received:2021-08-12 Revised:2021-10-01 Online:2022-04-30 Published:2022-04-07
Contact: GUO Bao-ping
Supported by:
Natural Science Foundation of Xinjiang Uygur Autonomous Region(2019D01C315)

摘要/Abstract

摘要：

目的了解新疆新源县人群多房棘球蚴病（AE）病例特征及其病原基因多态性情况,为制定AE预防控制策略提供参考。方法收集2016—2020年新疆医科大学第一附属医院生物样品库中新源县AE患者的病例信息及其手术切除的肝脏病灶样品,提取肝脏病灶样品基因组DNA,PCR扩增线粒体NADH脱氢酶2（nad2）基因并测序,PCR产物经测序剪接后进行BLAST比对,用MEGA10.0软件采用邻接法构建系统进化树,DnaSPv5软件分析扩增序列的基因型,Network10.0软件制作nad2基因型网络图。结果共收集AE病例28例,其中农区7例,巩乃斯河两岸流域21例;男性17例,女性11例,二者差异无统计学意义（χ² = 0.704,P > 0.05）;民族分布中,汉族12例、哈萨克族11例、维吾尔族3例、蒙古族1例、其他民族1例;职业分布中以农牧民为主,占92.86%（26/28）,其他职业仅占7.14%（2/28）,不同职业间病例数差异有统计学意义（χ² = 3.89,P < 0.05）;年龄分布中以20~49岁年龄组为主,占78.57%（22/28）,其他年龄组占21.43%（6/28）,不同年龄组间病例数差异无统计学意义（χ² = 0.418,P > 0.05）。25份多房棘球蚴肝组织病灶样品PCR扩增出大小约1 031 bp的片段,与预期大小一致,另3份未扩增出条带。序列比对结果显示,25条序列分属H1、H2、H3和H4等4个基因型,其中H1为主要基因型（占72.0%,18/25）,与哈萨克斯坦（GenBank登录号AB461406）分离株序列一致性为100%。系统进化树结果显示,新源县病例分离株的4个基因型与波兰（GenBank登录号KY205700）和哈萨克斯坦（GenBank登录号AB461406）分离株在同一条分支上,但与法国（GenBank登录号AB461404）、奥地利（GenBank登录号AB461403）、加拿大（GenBank登录号JF751036）、美国阿拉斯加（GenBank登录号MT429275）、美国印第安纳（GenBank登录号AB461409）及中国内蒙古（GenBank登录号AB461411）分离株亲缘关系较远。基因型网络图结果显示,以H1为中心,H2、H3、H4、中国四川,波兰、加拿大及日本分离株呈散射状分布,表明这些分离株均与H1亲缘关系较近,但与法国、奥地利、加拿大,美国阿拉斯加等序列不在同一分支,亲缘关系较远。结论本研究分析的28例AE患者主要为汉族和哈萨克族,农牧民占比较高,AE患者样品共有4个基因型,其中H1属新源县主要基因型。

关键词: 多房棘球蚴病, 新源县, nad2基因, 基因型

Abstract:

Objective To understand the characteristics of alveolar echinococcosis (AE) cases and the genetic polymorphism of the parasites in Xinyuan County, Xinjiang, to provide reference information for formulating developing prevention and control strategies for AE. Methods During 2016—2020, information on AE patients and some surgically resected liver lesion samples from Xinyuan County in the biological sample bank of the First Affiliated Hospital of Xinjiang Medical University were collected. Genomic DNA of the liver lesion samples was extracted, and the mitochondrial NADH dehydrogenase 2 (nad2) gene was amplified by PCR and sequenced. The PCR products were sequenced and spliced for Blast comparison. Mega10.0 software was used to construct a phylogenetic tree by the neighbor-joining method, DnaSPv5 software was used to analyze the genotype of amplified sequence, and Network10.0 software was used to make nad2 genotype network map. Results A total of 28 AE cases were collected, including 7 in rural areas and 21 in Kunes River Basin, among which 60.71% (17/28) were male, and 39.29% (11/28) were female (χ² = 0.704, P < 0.05). Ethnic distribution was mainly Han (42.86%, 12/28) and Kazak (39.29%, 11/28), and the highest infection rate among Mongolians was 11.17%. There was a significant difference in the infection rate among different ethnic groups (χ² = 61.08, P < 0.01). Farmers and herdsmen were the dominant occupations, accounting for 92.86% (26/28), while only 7.14% (2/28) were of other occupations. There was no significant difference in infection rate among different age groups (χ² = 0.418, P > 0.05). A expected, 25 samples were amplified by PCR with a fragment size of 1 031 bp. Sequence alignment results showed that there were four genotypes: H1, H2, H3 and H4, among which H1 was the main genotype and the sequence consistency was 100% with Kazakhstan (GenBank accession no. AB461406). Phylogenetic tree results showed that the four genotypes in Xinyuan county isolates were in the same branch as the Poland (GenBank accession no. KY205700) and Kazakhstan (GenBank accession no. AB461406), but more distantly related to France (GenBank Accession no. AB461404), Austria (GenBank accession no. AB461403), Canada (GenBank accession no. JF751036), Alaska (GenBank accession no MT429275), Indiana, USA (GenBank accession no. AB461409) and Inner Mongolia, China (GenBank accession no. AB461411). According to the results of centering on the H1, the genotype network diagram showed H2, H3, H4, Sichuan of China, Poland, Canada and Japan were scattered distributed which indicated that the isolates with H1 has a very close relationship, but it is not in the same clade with France, Austria, Canada, Alaska, USA isolates, and had a distant relationship. Conclusion The majority of AE patients were Han and Kazakian farmers and herdsmen, and the Mongolians had the highest infection rate. There were 4 genotypes in AE patients, among which H1 was the main genotype in Xinyuan County.

Key words: Alveolar echinococcoisis, Xinyuan County, Nad2 gene, Haplotype

中图分类号:

R532.32

才仁, 任远, 米荣升, 郭刚, 齐文静, 张壮志, 郭宝平. 新疆新源县多房棘球蚴病病例特征及其病原基因多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(2): 181-186.

CAI Ren, REN Yuan, MI Rong-sheng, GUO Gang, QI Wen-jing, ZHANG Zhuang-zhi, GUO Bao-ping. Characteristics of alveolar echinococcosis cases and genetic polymorphism of the parasite from Xinyuan County, Xinjiang[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2022, 40(2): 181-186.

图/表 3

参考文献 23

[1]	Deplazes P,, Rinaldi L,, Alvarez Rojas CA, et al. Global distribution of alveolar and cystic echinococcosis[J]. Adv Parasitol, 2017, 95: 315-493.
[2]	Nahorski WL,, Knap JP,, Pawlowski ZS, et al. Human alveolar echinococcosis in Poland: 1990-2011[J]. PLoS Negl Trop Dis, 2013, 7(1): e1986.
[3]	Li LH,, Wang W,, Hou XL, et al. Affects of Echinococcus multilocularis metacestode infection on the natural killer T cells and their subsets in mouse spleen[J]. Chin J Parasitol Parasit Dis, 2021, 39(3): 311-317. (in Chinese)
	(李玲慧,, 王慧,, 侯昕伶, 等. 多房棘球蚴感染对小鼠脾自然杀伤T细胞及其亚群的影响[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(3): 311-317.)
[4]	Guo BP. Study on correlation between pathogenic differences and mitochondrial genetic markers in Echinococcus multilocularis[D]. Shihezi: Shihezi University, 2019: 1-3. (in Chinese)
	(郭宝平. 多房棘球绦虫致病差异与线粒体遗传标志相关性的研究[D]. 石河子:石河子大学, 2019: 1-3.)
[5]	Li WD,, Wen H,, Hou J, et al. Role of extracellular matrix protein 1 in the liver fibrosis induced by Echinococcus multilocularis infection in mice[J]. Chin J Parasitol Parasit Dis, 2021, 39(3): 296-303. (in Chinese)
	(李文定,, 温浩,, 侯娇, 等. 细胞外基质蛋白1在多房棘球蚴感染小鼠肝纤维化过程中的作用[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(3): 296-303.)
[6]	Wen H,, Vuitton L,, Tuxun T, et al. Echinococcosis: advances in the 21st century[J]. Clin Microbiol Rev, 2019, 32(2): e00075-18.
[7]	Zhang C,, Shao Y,, Yang S, et al. T-cell tolerance and exhaustion in the clearance of Echinococcus multilocularis: role of inoculum size in a quantitative hepatic experimental model[J]. Sci Rep, 2017, 7(1): 3424. doi: 10.1038/s41598-017-03427-z
[8]	Guo B,, Zhang Z,, Guo Y, et al. High endemicity of alveolar echinococcosis in Yili Prefecture, Xinjiang Autonomous Region, the People’s Republic of China: infection status in different ethnic communities and in small mammals[J]. PLoS Negl Trop Dis, 2021, 15(1): e0008891. doi: 10.1371/journal.pntd.0008891
[9]	Nakao M,, Yokoyama N,, Sako Y, et al. The complete mitochondrial DNA sequence of the cestode Echinococcus multilocularis (Cyclophyllidea ∶ Taeniidae)[J]. Mitochondrion, 2002, 1(6): 497-509. doi: 10.1016/S1567-7249(02)00040-5
[10]	Wei YH,, Liu H,, Li WJ, et al. Analysis nad1 gene polymorphism of Echinococcus granulosus isolates from humans in Ali region of Tibet[J]. Chin J Parasitol Parasit Dis, 2020, 38(1): 17-21. (in Chinese)
	(魏玉环,, 刘华,, 李武军, 等. 西藏阿里地区细粒棘球蚴人体分离株nad1基因多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1): 17-21.)
[11]	Vanhove MPM,, Briscoe AG,, Jorissen MWP, et al. The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes ∶ Gyrodactylidae and Dactylogyridae)[J]. BMC Genomics, 2018, 19(1): 520. doi: 10.1186/s12864-018-4893-5
[12]	Guo B,, Zhang Z,, Zheng X, et al. Prevalence and molecular characterization of Echinococcus granulosus sensu stricto in northern Xinjiang, China[J]. Korean J Parasitol, 2019, 57(2): 153-159. doi: 10.3347/kjp.2019.57.2.153
[13]	Staubach C,, Thulke HH,, Tackmann K, et al. Geographic information system-aided analysis of factors associated with the spatial distribution of Echinococcus multilocularis infections of foxes[J]. Am J Trop Med Hyg, 2001, 65(6): 943-948. pmid: 11792003
[14]	Israyin U,, Tong SX,, Meng H, et al. Epidemiological survey of echinococcosis in Xinjiang in 2003[J]. Endem Dis Bull, 2009, 24(1): 65-67. (in Chinese)
	(伊斯拉音·乌斯曼,, 童苏祥,, 孟贺巴特, 等. 2003年新疆包虫病流行病学调查报告[J]. 疾病预防控制通报, 2009, 24(1): 65-67.)
[15]	Maimaitijiang W,, Adili S,, Yisilayin O, et al. Epidemiological survey of echinococcosis in Xinjiang Uygur Autonomous Region in 2012[J]. Chin J Parasitol Parasit Dis, 2016, 34(3): 249-254. (in Chinese)
	(买买提江·吾买尔,, 阿迪力·司马义,, 伊斯拉音·乌斯曼, 等. 2012年新疆维吾尔自治区人群棘球蚴病流行病学调查[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(3): 249-254.)
[16]	Gao YS,, Zhu MB,, Guo YZ, et al. Clinical analysis on hepatic hydatid disease in Yili River Valley[J]. Chin J Parasitol Parasit Dis, 2016, 23(1): 3-13. (in Chinese)
	(高永盛,, 朱马拜,, 郭永忠, 等. 新疆伊犁河谷肝棘球蚴病临床资料分析[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(1): 3-13.)
[17]	Karamon J,, Stojecki K,, Samorek-Pierog M, et al. Genetic diversity of Echinococcus multilocularis in red foxes in Poland: the first report of a haplotype of probable Asian origin[J]. Folia Parasitol (Praha), 2017, 9: 64.
[18]	Nakao M,, Xiao N,, Okamoto M, et al. Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis[J]. Parasitol Int, 2009, 58(4): 384-389. doi: 10.1016/j.parint.2009.07.010
[19]	Otero-Abad B,, Torgerson PR. A systematic review of the epidemiology of echinococcosis in domestic and wild animals[J]. PLoS Negl Trop Dis, 2013, 7(6): e2249. doi: 10.1371/journal.pntd.0002249
[20]	Wu C,, Zhang W,, Ran B, et al. Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China[J]. Parasit Vectors, 2017, 10(1): 265. doi: 10.1186/s13071-017-2172-y
[21]	Jia WZ,, Yan HB,, Guo AJ, et al. Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance[J]. BMC Genomics, 2010, 11: 447. doi: 10.1186/1471-2164-11-447
[22]	Torgerson PR,, Keller K,, Magnotta M, et al. The global burden of alveolar echinococcosis[J]. PLoS Negl Trop Dis, 2010, 4(6): e722. doi: 10.1371/journal.pntd.0000722
[23]	Torgerson PR,, Schweiger A,, Deplazes P, et al. Alveolar echinococcosis: from a deadly disease to a well-controlled infection. Relative survival and economic analysis in Switzerland over the last 35 years[J]. J Hepatol, 2008, 49(1): 72-77. doi: 10.1016/j.jhep.2008.03.023 pmid: 18485517

新疆新源县多房棘球蚴病病例特征及其病原基因多态性分析

Characteristics of alveolar echinococcosis cases and genetic polymorphism of the parasite from Xinyuan County, Xinjiang

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