CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2019 , Vol. 37 >Issue 3: 321 - 325

DOI: https://doi.org/10.12140/j.issn.1000-7423.2019.03.014

ORIGINAL ARTICLES

Infection and genotype of Giardia lamblia among HIV/AIDS patients in Guangxi

  • Hua LIU ,
  • Ning XU ,
  • Yu-juan SHEN ,
  • Yuan HU ,
  • Jian-ping CAO
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  • National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention;Chinese Center for Tropical Diseases Research;WHO Collaborating Centre for Tropical Diseases;National Center for International Research on Tropical Diseases, Ministry of Science and Technology;Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China

Received date: 2019-04-01

  Online published: 2019-07-10

Supported by

Supported by the National Key R&D Program of China (No. 2017YFD0500400, No. 2016YFC1201900）, Shanghai Municipal Commission of Health and Family Planning (No. 20164Y0225）, the National Science and Technology Major Program of China（No. 2018ZX10713001, No. 2018zx10102001-002-004）, the Project Sponsored by the Young Scholar Scientific Research Foundation of China CDC (No. 2016A102)

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Abstract

Objective To investigate the co-infection rate and the genotype of Giardia lamblia among HIV/AIDS patients in Guangxi Autonomous Region. Methods Fecal specimens were collected from 285 HIV/AIDS patients and 303 HIV-negative people from Guangxi Autonomous Region. DNA were extracted from the feces and triosephosphate isomerase (TPI) gene of G. lamblia was amplified by nested PCR to determine the infection of G. lamblia. The amplified TPI PCR products were sequenced and the obtained sequences were aligned and analyzed by BLAST search. Results The overall co-infection rate of G. lamblia with HIV/AIDS was 2.8% (8/285). There was no G. lamblia infection detected in people without HIV-AIDS (P < 0.01). However, no significant risk factors were found to be related to the infection of G. lamblia among HIV-AIDS patients such as age, gender, sanitation, antiretroviral treatment or clinical manifestations (diarrhea, CD4+T lymphocyte level). Seven samples were identified to be zoonotic assemblage B and 6 had 100% sequence homology with the reported human-derived isolate. The other isolate was assemblage C and sequence identical to the reported human-derived isolate in our previous study. Conclusion HIV-AIDS patients have higher co-infection rate with G. lamblia than people without HIV infection. The major genotype of infected G. lamblia was assemblage B.

Key words： Giardia lamblia; Assemblage; Nested PCR; HIV/AIDS

Cite this article

Hua LIU , Ning XU , Yu-juan SHEN , Yuan HU , Jian-ping CAO . Infection and genotype of Giardia lamblia among HIV/AIDS patients in Guangxi[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019 , 37(3) : 321 -325 . DOI: 10.12140/j.issn.1000-7423.2019.03.014

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