The Criteria for Detection of Malaria Parasite Nucleic Acid by Multiplex PCR Methods (T/SPMA 004-2023) (referred to as the Criteria) was compiled following the Social Organization Standardization-Part 1: Guidelines for Good Practice (GB/T 20004.1-2016) and Standardization Working Guidelines (GB/T 1.1-2009). The Criteria is composed of seven chapters, including “the range of application” “normative reference documents” “terms and definitions” “instruments and equipment” “reagent and materials” “detection steps” and “waste disposal and pollution prevention measures”. One informative appendix (technical principle) and four normative appendices (two detection methods, reagent preparation and sample preparation). The Criteria was issued by Shanghai Preventive Medicine Association through No.34 of SJPM in 2023. The Criteria provides for the technical reference for normative operations and the quality of sample testing in disease control institutions and medical institutions. And the Criteria makes up for the deficiency of specific operating steps in nucleic acid detection of Criteria for Diagnosis of Malaria (WS 259-2015).