CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2023 , Vol. 41 >Issue 3: 325 - 330

DOI: https://doi.org/10.12140/j.issn.1000-7423.2023.03.010

ORIGINAL ARTICLES

Investigation of fecal parasite pathogens in domestic dogs based on high-throughput sequencing

  • XIE Yi ,
  • WANG Ying ,
  • WANG Xu ,
  • SHI Dandan ,
  • FU Meihua ,
  • LI Chunyang ,
  • WU Weiping ,
  • DAN Bazeli ,
  • LIAO Sa ,
  • ZHANG Kaige ,
  • DENG Xueying ,
  • GUAN Yayi
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  • 1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research); NHC Key Laboratory of Parasite and Vector Biology; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Shanghai 200025, China
    2 Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200023, China
    3 Garzê Tibetan Autonomous Prefecture Center for Disease Control and Prevention, Garzê 626000, Sichuan, China
    4 Sichuan Province Center for Disease Prevention and Control, Chengdu 610041, China

Received date: 2022-12-31

  Revised date: 2023-02-13

  Online published: 2023-06-20

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Abstract

Objective To investigate the parasitic infection in domestic dogs in Da Hinggan Ling area, and provide reference for the prevention and control of local zoonotic parasitic diseases. Methods In September 2020, fresh domestic dog feces were collected from dog owner’s household yard of 6 natural villages including Jiabei Village, Baihua Village, Dongshan Village, Tuqiang Town, Xingfu Village and Guyuan Town in the Da Hinggan Ling area, and total DNA was extracted from the fecal samples. PCR amplification was conducted using 13 pairs of parasite universal primers targeting 8 taxonomy groups including Apicomplexa, Amoeba, Diplomonadida, Kinetoplastida, Parabasalia, Nematoda, Platyhelminthes and Microsporidia, and the target gene fragments were obtained after high-throughput sequencing of the amplified products. The product sequences were compared and analyzed in the NCBI database to identify the parasite species, and the detection rate of parasites in domestic dog feces was calculated. Results Parasite DNA were detected in 37 of 202 domestic dog feces, and the total detection rate was 18.31%. A total of 19 species of parasites which belongs to 15 families from 7 phyla were detected, and the detection rate of different species were significantly different (χ2 = 69.488, P < 0.05). Thirteen protozoa species and 6 helminth species were detected. The detection rate of Parabodo caudatus in the family Parabodonidae was the highest at 6.44% (13/202). Among the 37 samples in which parasite DNA was detected, the mixed positive ratio was 32.43% (12/37), of which 72.97% (27/37) detected 1 type of parasite, and 2 types, 3 kinds and 4 kinds accounted for 27.03% (10/37), 0.27% (1/37) and 0.27% (1/37) respectively. Jiabei Village had the highest detection rate of parasite DNA in domestic dog feces, which was 37.70% (23/61), followed by Tuqiang Town, with a detection rate of 36.36% (20/55), and Baihua Village, which was not detected (0/13). There was a statistically significant difference in the detection rate of different sampling sites (χ2 = 19.717, P < 0.05). Conclusion In Da Hinggan Ling area, domestic dogs were found infected with multiple parasites, showing considerably higher positive rate detected in some parts of the area, thus, there exists risk of infection caninee-source parasites to local residents.

Key words： Parasites; Domestic dogs; High-throughput sequencing; Zoonotic parasitic disease

Cite this article

XIE Yi , WANG Ying , WANG Xu , SHI Dandan , FU Meihua , LI Chunyang , WU Weiping , DAN Bazeli , LIAO Sa , ZHANG Kaige , DENG Xueying , GUAN Yayi . Investigation of fecal parasite pathogens in domestic dogs based on high-throughput sequencing[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023 , 41(3) : 325 -330 . DOI: 10.12140/j.issn.1000-7423.2023.03.010

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