CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2023 , Vol. 41 >Issue 1: 121 - 124

DOI: https://doi.org/10.12140/j.issn.1000-7423.2023.01.020

SHORT COMMUNICATIONS

Analysis of Blastocystis hominis infection in long-term hospitalized patients in Tianmen City

  • ZHU Mingchao ,
  • ZHU Ya ,
  • ZHAO Jianzhong ,
  • YUAN Huizhen
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  • 1. Department of Clinical Laboratory, the First people’s Hospital of Tianmen, Tianmen 431700, Hubei, China
    2. Department of Neurology, the First people’s Hospital of Tianmen, Tianmen 431700, Hubei, China
    3. Department of Clinical Laboratory, the First people’s Hospital of Xiangyang, Xiangyang 441000, Hubei, China
    4. College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China

Received date: 2022-05-05

  Revised date: 2022-06-16

  Online published: 2023-02-27

Supported by

Fundamental Research Funds for Central Universities of Wuhan University(2042020kfxg05)

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Abstract

Basic information (gender, age, fecal characteristics, occult-blood test results, clinical signs and symptoms, etc) of long-term hospitalized patients in Tianmen First People’s Hospital during June—September 2021 collected. The fecal samples of patients were collected and examined under microscope by direct smear with normal saline. The DNA were extracted from the suspected Blastocystis hominis positive fecal samples. Specific primers of B. hominis BhRDr and RD5 were used to amplify the target bands, and the positive fecal samples were sent to Norsay Genomics Research Center for sequencing. Giardia lamblia and fragile bicarbonate amoeba infection were also observed in the fecal samples of the patients. Anticoagulant whole blood and non-anticoagulant venous blood were collected for blood analysis. Among the 857 patients, 42 cases were positive for B. hominis, with a positive rate of 4.90%. The positive rate of males was 4.27% (19/445) and that among the females was 5.58% (23/412). There was no significant difference between the gender groups (χ2 = 0.791, P < 0.05). In different age groups, the positive rate was the highest in the ≥ 61 years old group (6.38%, 22/345). There was no significant difference in positive rates among different age groups (χ2 = 2.700, P > 0.05). Among different fecal characters, the positive rate of the loose stool samples was the highest (18.37%, 27/147) (χ2 = 98.930, P < 0.05). The positive rate was 6.72% (9/134) in patients with hematochezia and 4.56% (33/723) in patients without hematochezia, with no significant difference (χ2 = 1.123, P > 0.05). Among different clinical symptoms, patients with abdominal pain and diarrhea had the highest positive rate (4/11) (χ2 = 53.956, P < 0.05). Six patients were infected with B. hominis mixed with G. Lamblia, and two were infected with amoeba fragile. The red blood cell count and hemoglobin of B. hominis infected patients were (3.97 ± 7.39) × 1012/L and (119.02 ± 21.49) g/L, both of which were lower than that of the non-infected patients [(4.27 ± 0.44) × 1012/L, (126.52 ± 14.30) g/L] (t = 2.556, 2.235; P < 0.05). There were four genotypes of B. hominis (ST1, ST3, ST6 and ST7), and ST3 was the dominant one, accounting for 57.1% (24/42).

Key words： Blastocystis hominis; Fecal traits; Occult blood; Co-infection

Cite this article

ZHU Mingchao , ZHU Ya , ZHAO Jianzhong , YUAN Huizhen . Analysis of Blastocystis hominis infection in long-term hospitalized patients in Tianmen City[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023 , 41(1) : 121 -124 . DOI: 10.12140/j.issn.1000-7423.2023.01.020

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