CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2021 , Vol. 39 >Issue 1: 55 - 60

DOI: https://doi.org/10.12140/j.issn.1000-7423.2021.01.008

ORIGINAL ARTICLES

Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City

  • Ke QIAN ,
  • Zhu-hua HU ,
  • Xiu-ping WU ,
  • Guo-hua PENG ,
  • Ying-fang YU ,
  • Yan-hong CHU ,
  • Xian-feng ZHOU ,
  • Dan XU ,
  • Xue-jiao TENG ,
  • Hui LI ,
  • Li-guang TIAN
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  • 1 The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-origin and Vector-borne Diseases, Nanchang Center for Disease Control and Prevention, Nanchang 330038, China
    2 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Chinese Center for Tropical Diseases Research; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology; Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China
    3 Nanchang Municipal Health Commission, Nanchang 330038, China

Received date: 2020-09-28

  Revised date: 2020-11-25

  Online published: 2021-03-10

Supported by

National Natural Science Foundation of China(81473022);Scientific Research Project of Shanghai Municipal Health and Family Planning Commission(291440499);National Key Research and Development Program(2016YFC1202700);National Key Research and Development Program(2016YFC1202701);Science and Technology Program of Jiangxi Provincial Health and Family Planning Commission(20186023);Key R&D Project of Jiangxi Provincial Department of Science and Technology(20192BBGL70008)

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Abstract

Objective To determine the prevalence and genotype of Blastocystis hominis among HIV-positive individuals in Nanchang City. Methods A cross-sectional questionnaire survey was made among registered HIV-positive individuals in Nanchang City during May and September in 2016. Blood and fecal samples were collected. The level of CD4 + T cells in blood was detected. DNA genome was extracted from the fecal samples to amplify the small subunit ribosomal ribonucleic acid (SSU rDNA) of B. hominis. The positive products were sequenced and aligned to confirm the diagnosis, and phylogenic tree was constructed for genotyping. Results A total of 505 subjects were surveyed, and 505 fecal samples and 475 blood samples were collected. The infection rate of B. hominis was 4.16% (21/505) in the HIV/AIDS individuals, 4.22% (18/427) in the males and 3.85% (3/78) in the females, with no significant difference between males and females (P > 0.05). The infection rates in the HIV/AIDS populations from Methadone outpatient clinics of the Center for Disease Control, municipal infectious disease hospitals, and prison hospitals were 3.74% (4/107), 1.62% (5/308) and 13.33% (12/90), respectively. The infection rate in those from prison hospitals was significantly higher than the other two (P < 0.05). The infection rates in the HIV/AIDS populations with CD4 + T cell count > 400/μl and ≤ 400/μl were 3.29% (5/152) and 4.64% (15/323), respectively (P > 0.05). Sequencing results revealed prevalence of subtypes ST1 (4.76%, 1/21), ST3 (90.48%, 19/21), and ST7 (4.76%, 1/21) in Nanchang City. Conclusion There is high prevalence of B. hominis in HIV/AIDS cases from prison hospitals in Nanchang, and ST3 is the predominant subtype.

Key words： Blastocystis hominis; HIV/AIDS; Genotype characterization; Nanchang

Cite this article

Ke QIAN , Zhu-hua HU , Xiu-ping WU , Guo-hua PENG , Ying-fang YU , Yan-hong CHU , Xian-feng ZHOU , Dan XU , Xue-jiao TENG , Hui LI , Li-guang TIAN . Prevalence and genotype analysis of Blastocystis hominis among HIV/AIDS patients in Nanchang City[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021 , 39(1) : 55 -60 . DOI: 10.12140/j.issn.1000-7423.2021.01.008

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