CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >
Epidemiological investigation of paragonimiasis epidemic focus in Luoyang City of Henan Province
Received date: 2020-03-21
Online published: 2020-11-12
To investigate the status of Paragonimus spp. infection in freshwater crabs in Luoyang City of Henan Province, and identify the epidemic focus of paragonimiasis. Freshwater crabs as the second intermediate host were collected from surveillance sites in 9 counties(cities) of Luoyang City during the time from August 2013 to October 2019. Paragonimus spp. infection in the crabs was detected in laboratory settings. Paragonimus spp. metacercariae were identified by PCR amplification of the sequence of internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome oxidase subunit 1(CO1). A total of 560 freshwater crabs were captured, and Paragonimus metacercariae were found in the crabs from Yiyang County, Luoning County, Xin’an County, Song County, and Luanchuan County, and identified as the species P. skrjabini by morphological observation and PCR amplification. The infection rate and infectivity were found highest in the crabs collected from Yiyang County (58.33%, 11.54 metacercariae/crab), followed by Xin’an County (25.93%, 0.33 metacercariae/crab), Song County (19.64%, 0.23 metacercariae/crab), Luoning County (11.96%, 0.12 metacercariae/crab), and Luanchuan County (6.17%, 0.06 metacercariae/crab). Results of PCR showed that the ITS2 amplification product has a sequence length of 500 bp from the metacercariae isolated from the crabs of Yiyang County, Xin’an County, Songxian County, Luoning County and Luanchuan County, with 98%-100% homology to P. skrjabini (GenBank accession No. KX129924.1). It was recognized that five counties including Yiyang County, Luoning County, Xin’an County, Luanchuan County, and Song County were identified to be the epidemic foci of paragonimiasis among them, Yiyang County was a moderate endemic area, while the others being mild endemic regions.
Key words： Paragonimus; Freshwater crabs; Endemic focus; Investigation; Luoyang
Yun-xia LI , Lin AI , Yan DENG , Wei-qi CHEN , Xin ZHU , Zhi-guo YANG , Ke-wei LI , Yi-ying SUN . Epidemiological investigation of paragonimiasis epidemic focus in Luoyang City of Henan Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020 , 38(5) : 664 -666 . DOI: 10.12140/j.issn.1000-7423.2020.05.023
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