CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2020 , Vol. 38 >Issue 4: 449 - 453

DOI: https://doi.org/10.12140/j.issn.1000-7423.2020.04.009

ORIGINAL ARTICLES

Investigation of Fasciola spp. infection in Galba pervia and buffaloes in Guangxi

  • An-li LIANG ,
  • Zhen-ni NONG ,
  • Xiu-hong LU ,
  • Yun-liang SHI ,
  • Wei-yu ZHANG
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  • 1 Xiangsihu College of Guangxi University for Nationalities, Nanning 530008, China
    2 Guangxi Animal Epidemic Prevention and Control Center, Nanning 530021, China
    3 Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China
    4 College of Animal Science and Technology, Guangxi University, Nanning 530004, China

Received date: 2020-04-21

  Online published: 2020-09-09

Supported by

Science and Technology Major Project of Guangxi(桂科 No.AA17204057)

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Abstract

Objective To investigate the status of Fasciola spp. infection in the intermediate host Galba pervia and the definitive host buffaloes.Methods From April to October 2018, Galba pervia snails and buffalo feces were collected in six counties(cities) of Guangxi Province, including Hengxian County, Binyang County, Long’an County, Du’an County, Dahua County and Laibin County. The snails underwent compressed smear microscopy to observe worm larvae, and the fecal samples were examined using washing-sedimentation method for eggs. The DNA extracted from the larvae and eggs of Fasciola were processed for ITS2(internal transcribed spacer 2, ITS2) amplification and identification.Results A total of 1 523 snails were collected, showing an average Fasciola infection rate of 0.3% (5/1 523). Fasciola infection was found in the snail samples from four counties of Hengxian, Binyang, Long’an, and Laibin, but not from the Du’an and Dahua counties. A total of 128 buffalo fecal samples were collected, displaying an average Fasciola infection rate of 79.7% (102/128). The buffaloes from all six counties were found Fasciola egg positive in feces, with the Laibin County having the highest infection rate(88.2%, 15/17), and Du’an County the lowest rate (64.0%, 16/25). The sequence length of ITS2 amplification products was about 500 bp. The ITS2 amplification product from larvae in the snails had sequence homology of 98.8%-99.0% to that of F. gigantica (GenBank accession no. KF667375.1); the amplified sequence from fecal eggs of water buffaloes showed 98.4%-99.4% similarity to that of F. gigantica (GenBank accession no. KF667375.1).Conclusion The survey indicates that F. gigantica infections were found in snail Galba pervia and buffaloes in Guangxi, suggesting potential risk of human infection, thus, it is imperative to strengthen prevention and control measures.

Key words： Fasciola spp.; Galba pervia; Buffalo; Guangxi

Cite this article

An-li LIANG , Zhen-ni NONG , Xiu-hong LU , Yun-liang SHI , Wei-yu ZHANG . Investigation of Fasciola spp. infection in Galba pervia and buffaloes in Guangxi[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020 , 38(4) : 449 -453 . DOI: 10.12140/j.issn.1000-7423.2020.04.009

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