Investigation of Fasciola spp. infection in Galba pervia and buffaloes in Guangxi

doi:10.12140/j.issn.1000-7423.2020.04.009

Abstract

Abstract:

Objective To investigate the status of Fasciola spp. infection in the intermediate host Galba pervia and the definitive host buffaloes.Methods From April to October 2018, Galba pervia snails and buffalo feces were collected in six counties(cities) of Guangxi Province, including Hengxian County, Binyang County, Long’an County, Du’an County, Dahua County and Laibin County. The snails underwent compressed smear microscopy to observe worm larvae, and the fecal samples were examined using washing-sedimentation method for eggs. The DNA extracted from the larvae and eggs of Fasciola were processed for ITS2(internal transcribed spacer 2, ITS2) amplification and identification.Results A total of 1 523 snails were collected, showing an average Fasciola infection rate of 0.3% (5/1 523). Fasciola infection was found in the snail samples from four counties of Hengxian, Binyang, Long’an, and Laibin, but not from the Du’an and Dahua counties. A total of 128 buffalo fecal samples were collected, displaying an average Fasciola infection rate of 79.7% (102/128). The buffaloes from all six counties were found Fasciola egg positive in feces, with the Laibin County having the highest infection rate(88.2%, 15/17), and Du’an County the lowest rate (64.0%, 16/25). The sequence length of ITS2 amplification products was about 500 bp. The ITS2 amplification product from larvae in the snails had sequence homology of 98.8%-99.0% to that of F. gigantica (GenBank accession no. KF667375.1); the amplified sequence from fecal eggs of water buffaloes showed 98.4%-99.4% similarity to that of F. gigantica (GenBank accession no. KF667375.1).Conclusion The survey indicates that F. gigantica infections were found in snail Galba pervia and buffaloes in Guangxi, suggesting potential risk of human infection, thus, it is imperative to strengthen prevention and control measures.

Key words: Fasciola spp., Galba pervia, Buffalo, Guangxi

CLC Number:

R532.29

LIANG An-li, NONG Zhen-ni, LU Xiu-hong, SHI Yun-liang, ZHANG Wei-yu. Investigation of Fasciola spp. infection in Galba pervia and buffaloes in Guangxi[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(4): 449-453.

Figures/Tables 6

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调查地点 Investigated sites	小土蜗采集数 No. of Galba pervia	阳性小土蜗数 No. of positive Galba pervia	小土蜗感染率/% Infection rate in Galba pervia/%	水牛粪样采集数 No. of buffalo fecal samples	阳性水牛粪样数 No. of positive buffalo fecal samples	水牛感染率/% Infection rate in buffaloes/%
横县Heng County	320	1	0.3	17	14	82.3
宾阳县Binyang County	230	1	0.4	16	14	87.5
隆安县Long’an County	226	1	0.4	25	20	80.0
都安县Du’an County	158	0	0	25	16	64.0
大化县Dahua County	282	0	0	28	23	82.1
来宾Laibin City	307	2	0.7	17	15	88.2
合计	1 523	5	0.3	128	102	79.7