CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2020 , Vol. 38 >Issue 2: 255 - 258

DOI: https://doi.org/10.12140/j.issn.1000-7423.2020.02.021

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Affects of Plasmodium berghei infection in mice on spleen B cells and natural killer cells and their surface molecules

  • Jie-sen ZHANG ,
  • Meng-xin ZHANG ,
  • Meng-yi HAN ,
  • Bing-xia CHEN ,
  • Yong-sen LI ,
  • Chen-xi JIN ,
  • Yan-wei QI
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  • 1 The Third Clinical College, Guangzhou Medical University, Guangzhou 511436, China
    2 Department of Pathogenic Biology and Immunology, Guangzhou Medical University, Guangzhou 511436, China

Received date: 2019-09-26

  Online published: 2020-05-11

Supported by

Supported by the National Natural Science Foundation of China(81902087);Guangdong Natural Science Fund Project(2017A030310535);Guangdong Natural Science Fund Project(No. S201910570023);Guangdong University Student Innovation and Entrepreneurship Training Program(S201910570027);Guangzhou Medical University Student Innovation and Entrepreneurship Training Project(2018A036);Guangzhou Medical University Third Clinical College University Student Scientific Research Project(2018A0024);Guangzhou Medical University Third Clinical College University Scientific Research Project(2018A0020)

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Abstract

To investigate the affects of Plasmodium berghei infection on spleen B cells and NK cells as well as their surface molecules in mice, 10 female C57BL/6 mice were randomly assigned into control group and infection group, five mice each. The mice in the infection group were infected with P. berghei by injection through the tail vein (10 6 parasites/mouse), while the control group received the same volume of saline. Six days after infection, spleens were collected to prepare single cell suspensions, and using flow cytometry to assay the amount of spleen B cells and NK cells and the expression of their surface molecules CD62L, CXCR3 and CD69. The results showed that the percentage of B cells in the infection group was (79.2 ± 3.6)%, significantly higher than that in the control group [(54.3 ± 4.4)%, P < 0.01]. The amount percentage of spleen NK cells in the infection group was (2.2 ± 0.7)%, significantly lower than that in the normal control group [(4.6 ± 0.8)%, P < 0.01]. The percentage of CD62L expression on B cells in the infection group was (77.7 ± 4.4)%, higher than that in the normal group (72.8 ± 7.1)%, but with no statistical significance (P > 0.05). The percentage of CD62L expression on NK cells in the infection group was (61.9 ± 4.8)%, which was significantly lower than that in the control group [(86.6 ± 4.2)%, P < 0.01]. The percentage of CXCR3 expression on B cells in the infection group was (4.1 ± 0.1)%, which was significantly higher than that in the control group [(3.0 ± 0.2) %, P < 0.01]. The percentage of CXCR3 expression on NK cells was (2.1 ± 0.9)%, which was lower than that of the control group [(2.3 ± 0.4)%], but with no statistical significance (P > 0.05). The percentages of CD69 expression on B cells and NK cells were (54.3 ± 4.7)% and (22.2 ± 1.6)%, respectively, both significantly higher than those in the control group [(1.9 ± 0.4)%, (1.3 ± 0.3)%, P < 0.01]. These results suggest that the mice infected with P. berghei may exert the affect against the infection through increasing B cell recruitment and up-regulating the expression of their surface molecules CXCR3 and CD69, and NK cell surface molecule CD69.

Key words： Plasmodium berghei; Spleen; Immune cells; Cell surface molecules

Cite this article

Jie-sen ZHANG , Meng-xin ZHANG , Meng-yi HAN , Bing-xia CHEN , Yong-sen LI , Chen-xi JIN , Yan-wei QI . Affects of Plasmodium berghei infection in mice on spleen B cells and natural killer cells and their surface molecules[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020 , 38(2) : 255 -258 . DOI: 10.12140/j.issn.1000-7423.2020.02.021

References

[1] Yang LX, Zeng JF, Cai H , et al. Establishment of a PCR-based method for detection of malaria parasites and its application in screening of blood donors[J]. China Trop Med, 2006,6(10):1745-1746. (in Chinese)
[1] ( 杨立新, 曾劲峰, 蔡红 , 等. 疟原虫核酸检测技术建立及在献血员筛查应用的研究[J]. 中国热带医学, 2006,6(10):1745-1746.)
[2] Teo A, Feng GQ, Brown GV , et al. Functional antibodies and protection against blood-stage malaria[J]. Trends Parasitol, 2016,32(11):887-898.
[3] Li MY, Zhu XT . The role of IgG and its subclasses in malaria protective immunity[J]. Chin J Immunol, 2016,32(7):1074-1076. (in Chinese)
[3] ( 李明洋, 朱晓彤 . IgG及其亚类在疟疾保护性免疫中的作用[J]. 中国免疫学杂志, 2016,32(7):1074-1076.)
[4] McCall MB, Roestenberg M, Ploemen I , et al. Memory-like IFN-γ response by NK cells following malaria infection reveals the crucial role of T cells in NK cell activation by P. falciparum[J]. Eur J Immunol, 2010,40(12):3472-3477.
[5] Mavoungou E, Luty AJ, Kremsner PG . Natural killer (NK) cell-mediated cytolysis of Plasmodium falciparum-infected human red blood cells in vitro[J]. Eur Cytokine Netw, 2003,14(3):134-142.
[6] Ni JM, Ni AP . Research progress of regulation mechanism of innate immunity in malaria[J]. Chin Pharmacol Bull, 2018,34(5):612-615. (in Chinese)
[6] ( 倪杰明, 倪安平 . 疟疾的固有免疫调节机制相关研究进展[J]. 中国药理学通报, 2018,34(5):612-615.)
[7] Groom JR, Luster AD . CXCR3 ligands: redundant, collaborative and antagonistic functions[J]. Immunol Cell Biol, 2011,89(2):207-215.
[8] Feng YM, Chen QP, Huang LT , et al. Expression changes of CD62L, BAFF and CD40L in basophils of patients with systemic lupus erythematosus[J]. Shandong Med J, 2017,57(9):20-23. (in Chinese)
[8] ( 冯永民, 陈秋萍, 黄柳涛 , 等. 活动性系统性红斑狼疮患者外周血嗜碱性粒细胞CD62L、BAFF和CD40L表达变化及意义[J]. 山东医药, 2017,57(9):20-23.)
[9] Dong WL . Progress in research on obstructive sleep apnea hypopnea syndrome and oxidative stress[J]. Clin Med China, 2013,29(6):670-672. (in Chinese)
[9] ( 董万里 . 阻塞性睡眠呼吸暂停低通气综合征与氧化应激研究进展[J]. 中国综合临床, 2013,29(6):670-672.)
[10] Zhao X . Immune response of B cells induced by porcine epidemic diarrhea virus[D]. Baoding: Hebei Agricultural University, 2019. ( in Chinese)
[10] ( 赵雪 . 猪流行性腹泻病毒诱导的B细胞免疫应答研究[D]. 保定: 河北农业大学, 2019.)
[11] Matsushita T . Regulatory and effector B cells: Friends or foes[J]. J Dermatol Sci, 2019,93(1):2-7.
[12] Castillo-Méndez SI, Zago CA, Sardinha LR , et al. Characterization of the spleen B-cell compartment at the early and late blood-stage Plasmodium chabaudi malaria[J]. Scand J Immunol, 2007,66(2/3):309-319.
[13] Wang ZH, Liu HH, Ma N , et al. A preliminary study on the expression of CD160 on NK cells and its mechanism of mediating NK killing effect[J]. J Exp Hematol, 2018,26(5):1559-1564. (in Chinese)
[13] ( 王振华, 刘绘绘, 马宁 , 等. NK细胞表面CD160分子表达及其介导NK杀伤效应机制的初步研究[J]. 中国实验血液学杂志, 2018,26(5):1559-1564.)
[14] Yindom LM, Forbes R, Aka P , et al. Killer-cell immunoglobulin-like receptors and malaria caused by Plasmodium falciparum in The Gambia[J]. Tissue Antigens, 2012,79(2):104-113.
[15] Hansen DS, Bernard NJ, Nie CQ , et al. NK cells stimulate recruitment of CXCR3 + T cells to the brain during Plasmodium berghei-mediated cerebral malaria [J]. J Immunol, 2007,178(9):5779-5788.
[16] Ley K, Kansas GS . Selectins in T-cell recruitment to non-lymphoid tissues and sites of inflammation[J]. Nat Rev Immunol, 2004,4(5):325-335.
[17] Li QH, Wei L, Wang KQ . Progress in the application of CD69 molecule in γδΤ cells[J]. Chin J Biomed Engin, 2016,22(4):337-339. (in Chinese)
[17] ( 李清华, 魏丽, 王克强 . CD69分子在γδΤ细胞中的应用进展[J]. 中华生物医学工程杂志, 2016,22(4):337-339.
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