CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES >

2020 , Vol. 38 >Issue 2: 219 - 223

DOI: https://doi.org/10.12140/j.issn.1000-7423.2020.02.014

ORIGNAL ARTICLES

The developmental characteristics of Triatoma rubrofasciata under experimental conditions

  • Li-zhen XIAO ,
  • Han-guo XIE ,
  • Zhu-yun CHEN ,
  • Rong OUYANG ,
  • Dian-wei JIANG
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  • Fujian Center for Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China

Received date: 2019-11-04

  Online published: 2020-05-11

Supported by

Supported by the National Key Research and Development Program of China(2016YFC1202000);Supported by the National Key Research and Development Program of China(2016YFC1202003);National Parasite Germplasm Resource Sharing Service Platform(平台-TDRC-22)

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Abstract

Objective To investigate the developmental course of Triatoma rubrofasciata, in order to provide referential basis for its studies and control.Methods T. rubrofasciata were collected from Huping village of Hua’an county of Zhangzhou City, Fujiang Province, and maintained at laboratory conditions at 26℃ with relative humidity of 60%, during May 2018 to September 2019. With ICR mice as the only blood supplier(blood supplied for 3 days), the T. rubrofasciata were observed at various developmental stages on hatching, blood sucking, and molting.Results Under the designed experimental conditions, the T. rubrofasciata could completed the whole life history, including 7 stages covering egg stage, nymph stages Ⅰ-Ⅴ and adult stage, lasting for 117.7 days on average. The hatching rate of eggs was 92.2% (391/424). Both nymphs and adults suck blood, nymphs at all stages could not molt until they had sucked enough amount of blood, and female adults could not produce eggs unless they had suck blood. The blood-taking rate and molting rate of stage Ⅰ-Ⅴ nymphs were 86.4% (338/391) and 72.4% (283/391), 76.7% (217/283) and 58.3% (165/283), 71.5% (118/165) and 52.1% (86/165), 91.9% (79/86) and 64.0% (55/86), 96.4% (53/55) and 56.4% (31/55), respectively. The amount of blood taken by the nymphs increased with the growing stages, being (2.9 ± 11.0), (8.0 ± 3.0), (14.9 ± 8.8), (85.4 ± 17.8), and (101.6 ± 54.2) mg for stageⅠ-Ⅴ nymphs, and the folds of body weight gain after blood-feeding were 5.4 ± 1.8, 3.8 ± 1.2, 2.8 ± 1.1, 5.1 ± 1.4, and 2.6 ± 1.1, respectively. The average amount of blood taken was highest at stage V nymphs, and the fold of body weight gain was highest at stage I nymphs.Conclusion T. rubrofasciata can complete its life cycle by feeding animal blood under the experimental conditions, which provides an laboratory platform for related research.

Key words： Triatoma rubrofasciata; Developmental course; Amount of blood taken; Molting rate

Cite this article

Li-zhen XIAO , Han-guo XIE , Zhu-yun CHEN , Rong OUYANG , Dian-wei JIANG . The developmental characteristics of Triatoma rubrofasciata under experimental conditions[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020 , 38(2) : 219 -223 . DOI: 10.12140/j.issn.1000-7423.2020.02.014

References

[1] Pérez-Molina JA, Perez AM, Norman FF , et al. Old and new challenges in Chagas disease[J]. Lancet Infect Dis, 2015,15(11):1347-1356.
[2] World Health Organization. Chagas disease (American trypanosomiasis) [EB/OL].( 2018- 02- 01) [2018-09-28] .
[3] Carod-Artal FJ . American trypanosomiasis[J]. Handb Clin Neurol, 2013,114:103-123.
[4] Tarleton RL, Curran JW . Is Chagas disease really the “new HIV/AIDS of the Americas”[J]. PLoS Negl Trop Dis, 2012,6(10):e1861.
[5] Galv?o C, Justi SA . An overview on the ecology of Triatominae (Hemiptera: Reduviidae)[J]. Acta Trop, 2015,151:116-125.
[6] Coura JR . The main sceneries of Chagas disease transmission. The vectors, blood and oral transmissions: a comprehensive review[J]. Mem Inst Oswaldo Cruz, 2015,110(3):277-282.
[7] World Health Organization . Informal consultation on Chagas disease in the Western Pacific[R]. Nagasaki: WHO, 2011.
[8] Xiao CY, Ren SZ, Zhenmg LY , et al. Manual of Hemipterous Insects in China (Hemiptera-Heteroptera) (Secondly Volume) [M]. Beijing: Science Press, 1981: 390-439. (in Chinese)
[8] ( 萧采瑜, 任树芝, 郑乐怡 , 等. 中国蝽类昆虫鉴定手册(半翅目异翅亚目)(第二册)[M]. 北京: 科学出版社, 1981: 390-439.)
[9] Depickère S, Ravelo-García AG, Lardeux F . Chagas disease vectors identification using visible and near-infrared spectroscopy[J]. Chemom Intell Lab Syst, 2020,197:103914.
[10] Qian YJ, Li SZ, Wang Q , et al. Rapid risk assessment on the import of American trypanosomiasis to China[J]. Chin J Parasitol Parasit Dis, 2013,31(1):57-59. (in Chinese)
[10] ( 钱颖骏, 李石柱, 王强 , 等. 中国输入性美洲锥虫病疫情的快速风险评估[J]. 中国寄生虫学与寄生虫病杂志, 2013,31(1):57-59.)
[11] Wang LY, Peng LF . Four cases of anaphylactic shock caused by triatomine bugs[J]. Clin Focus, 2006,21(14):1059-1061. (in Chinese)
[11] ( 王立毅, 彭礼飞 . 锥蝽致过敏性休克4例[J]. 临床荟萃, 2006,21(14):1059-1061.)
[12] Liu Q, Guo YH, Zhang Y , et al. First records of Triatoma rubrofasciata (De Geer, 1773) (Hemiptera, Reduviidae) in Foshan, Guangdong Province, Southern China[J]. Infect Dis Poverty, 2017,6(1):129.
[13] Huang YL, Huang DN, Wu WH , et al. Identification and characterization of the causative triatomine bugs of anaphylactic shock in Zhanjiang, China[J]. Infect Dis Poverty, 2018,7(1):127.
[14] Hu Y, Gao MZ, Huang P , et al. Taxonomic integrative and phylogenetic identification of the first recorded Triatoma rubrofasciata in Zhangzhou, Fujian Province and Maoming, Guangdong Province, China[J]. Infect Dis Poverty, 2019,8(1):70.
[15] Justi SA, Galv?o C . The evolutionary origin of diversity in chagas disease vectors[J]. Trends Parasitol, 2017,33(1):42-52.
[16] Dujardin JP, Lam TX, Khoa PT , et al. The rising importance of Triatoma rubrofasciata[J]. Mem Inst Oswaldo Cruz, 2015,110(3):319-323.
[17] Galv?o C, Carcavallo R, Rocha DDS , et al. A checklist of the current valid species of the subfamily Triatominae Jeannel, 1919(Hemiptera, Reduviidae) and their geographical distribution, with nomenclatural and taxonomic notes[J]. Zootaxa, 2003,202(1):1-36.
[18] Braga MV, Lima MM . Feeding and defecation patterns of nymphs of Triatoma rubrofasciata (De Geer, 1773) (Hemiptera: Reduviidae), and its potential role as vector for Trypanosoma cruzi[J]. Mem Inst Oswaldo Cruz, 1999,94(1):127-129.
[19] Xiao LZ, Chen ZY, Ouyang R , et al. Morphological characteristics of Triatoma rubrofasciata at different developmental stages[J]. Chin J Parasitol Parasit Dis, 2018,36(6):605-610. (in Chinese)
[19] ( 肖丽贞, 陈朱云, 欧阳榕 , 等. 红带锥蝽各发育阶段形态特征观察[J]. 中国寄生虫学与寄生虫病杂志, 2018,36(6):605-610.)
[20] Zhu D, Liu Q, Zhang Y , et al. Laboratory rearing and observation on the biological characteristics of Triatoma rubrofasciata[J]. Chin J Vector Biol Control, 2019,30(3):281-285. (in Chinese)
[20] ( 朱丹, 刘琴, 张仪 , 等. 红带锥蝽的实验室人工饲养和部分生物学特性观察[J]. 中国媒介生物学及控制杂志, 2019,30(3):281-285.
[21] Association of Schools of Public Health. The Triatominae of north and central America and the west indies and their public health significance: a review[J]. Pub Health Reports 1945,60(35):1036.
[22] Zeledón R, Guardia VM, Zúniga A , et al. Biology and ethology of Triatoma dimidiata (Latreille, 1811). Ⅰ. Life cycle, amount of blood ingested, resistance of starvation, and size of adults[J]. J Med Entomol, 1970,7(3):313-319.
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