The developmental characteristics of Triatoma rubrofasciata under experimental conditions

doi:10.12140/j.issn.1000-7423.2020.02.014

Abstract

Abstract:

Objective To investigate the developmental course of Triatoma rubrofasciata, in order to provide referential basis for its studies and control.Methods T. rubrofasciata were collected from Huping village of Hua’an county of Zhangzhou City, Fujiang Province, and maintained at laboratory conditions at 26℃ with relative humidity of 60%, during May 2018 to September 2019. With ICR mice as the only blood supplier(blood supplied for 3 days), the T. rubrofasciata were observed at various developmental stages on hatching, blood sucking, and molting.Results Under the designed experimental conditions, the T. rubrofasciata could completed the whole life history, including 7 stages covering egg stage, nymph stages Ⅰ-Ⅴ and adult stage, lasting for 117.7 days on average. The hatching rate of eggs was 92.2% (391/424). Both nymphs and adults suck blood, nymphs at all stages could not molt until they had sucked enough amount of blood, and female adults could not produce eggs unless they had suck blood. The blood-taking rate and molting rate of stage Ⅰ-Ⅴ nymphs were 86.4% (338/391) and 72.4% (283/391), 76.7% (217/283) and 58.3% (165/283), 71.5% (118/165) and 52.1% (86/165), 91.9% (79/86) and 64.0% (55/86), 96.4% (53/55) and 56.4% (31/55), respectively. The amount of blood taken by the nymphs increased with the growing stages, being (2.9 ± 11.0), (8.0 ± 3.0), (14.9 ± 8.8), (85.4 ± 17.8), and (101.6 ± 54.2) mg for stageⅠ-Ⅴ nymphs, and the folds of body weight gain after blood-feeding were 5.4 ± 1.8, 3.8 ± 1.2, 2.8 ± 1.1, 5.1 ± 1.4, and 2.6 ± 1.1, respectively. The average amount of blood taken was highest at stage V nymphs, and the fold of body weight gain was highest at stage I nymphs.Conclusion T. rubrofasciata can complete its life cycle by feeding animal blood under the experimental conditions, which provides an laboratory platform for related research.

Key words: Triatoma rubrofasciata, Developmental course, Amount of blood taken, Molting rate

CLC Number:

R384.9

Li-zhen XIAO, Han-guo XIE, Zhu-yun CHEN, Rong OUYANG, Dian-wei JIANG. The developmental characteristics of Triatoma rubrofasciata under experimental conditions[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2020, 38(2): 219-223.

Figures/Tables 3

References 22

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