Screening of antigens specific for the anti-Cysticercus cellulosae IgG4 antibody

CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES ›› 2017, Vol. 35 ›› Issue (5): 509-511.

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Screening of antigens specific for the anti-Cysticercus cellulosae IgG4 antibody

Jin LI¹, Yan WANG², Qing-kuan WEI¹, Feng-ju JIA¹, Ting XIAO¹, Hui SUN¹, Zhen-hua YU¹, Bing-cheng HUANG^1,^*()

1 Shandong Institute of Parasitic Diseases, WHO collaborating Center for Lymphatic Filariasis and Taeniasis/Cysticercosis, Jining, 272033, China
2 Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China

Received:2017-04-11 Online:2017-10-30 Published:2018-01-08
Contact: Bing-cheng HUANG E-mail:hbc863@hotmail.com
Supported by:
Supported by the Natural Science Fund of Shandong Province(No. ZR2015YL033, ZR2015YL051), the National Natural Science Foundation of China(No. 30872208)and the Innovation Project of Shandong Academy of Medical Sciences

Abstract

Abstract:

The prepared cyst fluid antigen was separated by two-dimensional electrophoresis(2-DE). The protein spots were analyzed by ImageMaster 2D Plantinum 6.0 software after Coomassie blue staining, on which Western blotting analysis was performed using sera of patients with cerebral cysticercosis as the primary antibody. The positive protein spots were selected and analyzed by Matrix-assisted laser desorption ionization tandem mass spectrometry (GC-MS), and the peptide mass fingerprints were obtained. The specific Cysticercus cellulosae antigen for IgG4 antibody was identified with the Mascot software by searching and alignment in NCBI. 2-DE detected 201 ± 5 protein spots in the cystic acid antigen, with a relative molecular weight （M_r） of 10 000-170 000 and a pI of 3.0-10.0. Western blotting analysis showed that the antigens binding the specific IgG4 antibody were mainly small molecular-weight proteins. Fifty-one(antigen) spots were obtained from them for mass spectrometry, and 21 of them were positive(peak score>39). The positive spots were related with 10 cysticercus proteins, most closely with Ts8B2, Ts8B3, 10ku antigen and Sequence 3.

Key words: Cysticercus cellulosae, Cysticercus fluid antigens, IgG4, Two-dimensional electrophoresis

CLC Number:

R532.32

Jin LI, Yan WANG, Qing-kuan WEI, Feng-ju JIA, Ting XIAO, Hui SUN, Zhen-hua YU, Bing-cheng HUANG. Screening of antigens specific for the anti-Cysticercus cellulosae IgG4 antibody[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(5): 509-511.

Figures/Tables 2

References 16

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蛋白名称	NCBI检索ID	相对分子质量	等电点	分值
RS1	gi22657787	9217	8.9	45
Secreted antigen Ts8B3	gi23477226	7570	6.71	105
Cysticercosis 10ku antigen	gi112785160	9650.2	8.89	100
Secreted antigen Ts8B2	gi23477224	9680.2	9.15	115
Sequence 3 from patent US 6589752	gi33757615	9576.2	6.58	85
8 ku diagnostic antigen TsRS1	gi19879956	8641.7	8.91	41
Unnamed protein product	gi13185479	8271.4	8.9	41
Immunogenic protein Ts21	gi7339851	9671.3	9.23	40
Immunogenic protein	gi12802157	9458.2	9.23	40
RS1 variant	gi112819965	9589.2	9.22	39

Screening of antigens specific for the anti-Cysticercus cellulosae IgG4 antibody

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