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Efficacy of Capture and Ligation Probe-PCR with#br# Pooling Strategy in Detection of Plasmodium spp.

ZHOU Yao-wu1, LIN Zu-rui1, CHENG Zhi-bin2, ZHENG Zhi2，ZHOU Xing-wu1, ZHOU Zi-you1, WANG Duo-quan3, LI Jian-xiong1, ZENG Xu-can1, SUN Xiao-dong1*   

  1. 1 Yunnan Institute of Parasitic Disease, Yunnan Center for Malaria Research, Yunnan Key Laboratory of Vector-borne Diseases Control and Research, Pu’er 665000, China；2 Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Beijing 100005, China； 3 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention；WHO Collaborating Centre for Tropical Diseases；National Center for International Research on Tropical Diseases, Ministry of Science and Technology；Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China
  • Online:2016-08-30 Published:2016-11-07
  • Supported by:

    Supported by the Candidates of the Young and Middle Aged Academic Leaders of Yunnan Province（No. 2015HB075）

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Abstract:

The capture and ligation probe-PCR（CLIP-PCR） with pooling strategy method and microscopy were applied on 100 clinical samples（7 positive and 93 negative samples） from the malaria reference laboratory in Yunnan Province. By calculating the detection rate, sensitivity, specificity, detection time and detection cost, the efficacy of the CLIP-PCR with pooling strategy method in detecting Plasmodium spp. was evaluated. The CLIP-PCR with matrix pooling strategy successfully detected Plasmodium spp. in all the 7 positive samples. Its sensitivity and specificity relative to the microscopy as a gold standard were both 100%. The detection time for all the samples by CLIP-PCR was 5.0 h, 85.0% shorter than that by microscopy（33.3 h）, and the detection cost was 300 yuan, 75.0% less than that by microscopy （1 000 yuan）.

Key words: Capture and ligation probe-PCR, Pooling strategy, Evaluation