›› 2005, Vol. 23 ›› Issue (3): 5-149.

• 论著 • Previous Articles     Next Articles

Comparative Study on the Resting Habit of Anopheles minimus Aand Anopheles minimus C in Yunnan Province

ZHENG Bin;TANG Lin-hua;MA Ya-jun;SHI Wen-qi, ZHOU Shui-sen;WANG Xue-zhong   

  1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention，WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-06-30 Published:2005-06-30

PDF (PC)

Abstract: Objective To compare the difference of resting habitat between Anopheles minimus A and An. minimus C in Yunnan Province. Methods A village was selected in Mengla and Yuanjiang County respectively to collect mosquitoes by hanging ultraviolet lamps inside the cattle pen and human dwellings. The samples were identified by multiplex-PCR after morphological examination. Suspicious An. minimus A/C hybrid samples were identified by PCR-ASA, PCR-RFLP and D3 region sequence. Results In human dwellings, the proportion of An. minimus A and C was 21.35% and 78.65%, and in cattle pen, 28.54% and 71.46% respectively. As for the An. minimus A/C hybrid, the result of PCR-ASA and PCR-RFLP displayed the pattern of both An. minimus A and An. minimus C (PCR-ASA: 376、 294 and 112 bp, PCR-RFLP:108、 268 and 376 bp), the D3 sequence displayed heterozygosis in the variation place of An. minimus A and An. minimus C, which means that the hybrid possesses the signal of both An. minimus A and An. minimus C. Conclusion There is no resting habit difference between An. minimus A and An. minimus C in Yunnan Province. In Mengla County, 5 An. minimus A/C hybrids have been found for the first time.

Key words: Anopheles minimus, Resting habit, An. minimus A/C hybrid