Abstract: Objective   To determine the blood-sucking habit of anopheline by mosquitoes bloodmeal identification in malaria endemic area Chayu County, Tibet Autonomous Region.  Methods  Three villages with different bio-enviroments were selected as the investigation spots. Light traps were set up outdoor and in livestock sheds from sunset （20 ∶ 00） to sunrise （8 ∶ 00） in 3-4 consecutive nights to collect mosquitoes. The trapped anophelines were counted and identified according to morphological criteria and multiple PCR method. A PCR-based methodology according to the mtDNA-cytb variations was used in different mammal hosts to identify bloodmeal sources in engorged mosquitoes. The human blood index (HBI) was assessed to determine the range of hosts.  Results  Among 1 442 anopheline mosquitoes collected by 108 lighttraps on 13 nights. 1 436（99.6%） belonged to Anopheles maculatus complex, with 85.5% An. pseudowillmori and 14.5% An. willmori. Positive bloodmeal identification was found from 168（83%） of 202 field-collected engorged mosquitoes. The crude HBI of An. pseudowillmori and An. willmori were 0.35 and 0.29, respectively.  Conclusion  An. pseudowillmori and An. willmori are both zoophilic and anthropophilic, and An. pseudowillmori shows a higher HBI.

Key words: Tibet, Chayu county, Anopheles, Multi-PCR, Human blood index