Abstract: AIM: To compare the morphology of wing spot between Anopheles minimus Theobald, 1901 and Anopheles fluviatilis James, 1902 and find out the reliable diagnostic characteristics. METHODS: Anopheles minimus were collected from Jinuo Village, Jinghong County,Yunnan Province. Anopheles fluviatilis were collected from Lingyun County, Guangxi Zhuang Autonomous Region. Measurements of the wing length and the length of pale and dark spots on the costa and V1 were made with a microscope fitted with an ocular micrometer. RESULTS: A total of 52 females and 60 males of Anopheles minimus and 40 females and 60 males of Anopheles fluviailis were examined. It was found that the presence of presector pale spot and the ratio of sector pale spot to sector dark spot were the important diagnostic morphological characteristics between the above mentioned two species. CONCLUSION: obvious differentiations exist in the morphology of wing spots between Anopheles minimus and Anopheles fluviatilis

Key words: Anopheles minimus, Anopheles fluviatilis, wing spot, morphology