Abstract:

Specific primers were designed and synthesized based on the

reported glyceraldehydes-3-phosphate dehydrogenase（BmG3PD） gene of Brugia malayi（GenBank Accession No. U18137）. Total RNA was extracted from

Brugia malayi and its BmG3PD gene was amplified

by reverse transcription-polymerase chain raction（RT-PCR）. The PCR product was purified and cloned into plasmid pGEM-T, then transformed into Escherichia coli DH5α. The recombinant

plasmids were screened and identified by digestion

with restriction enzyme and PCR amplification. The positive recombinant plasmid pGEM-T-BmG3PD was confirmed by sequencing and homology comparison. Five parameters and

methods were used to predict B-cell epitopes in amino acid sequence of BmG3PD. The amplified DNA fragment（1 020 bp） had a high identity of 99% with the BmG3PD gene sequence of

Brugia malayi. B-cell epitopes of BmG3PD were probably at or adjacent to 22-36, 242-255, 303-318 and 326-336 in its amino acid sequence.

Key words: Brugia malayi, G3PD, Gene cloning, Sequence analysis, B-cell epitope