COMPARISON OF CIRCULATING ANTIGEN DETECTION IN SCHISTOSOMIASIS JAPONICA PATIENTS USING TWO METHODS OF ELISA 

›› 2000, Vol. 18 ›› Issue (4): 10-228.

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COMPARISON OF CIRCULATING ANTIGEN DETECTION IN SCHISTOSOMIASIS JAPONICA PATIENTS USING TWO METHODS OF ELISA 

QIU Li-zhu ZHANG Yong-hong LI Hao XUE Hai-chou

Institute of Parasitic Diseases**;Chinese Academy of Preventive Medicine;Shanghai 200025

Received:1900-01-01 Revised:1900-01-01 Online:2000-08-30 Published:2000-08-30

Abstract

Abstract: 　Objective] To compare 2 kinds of ELISA methods for detecting circulating antigen in schistosomiasis patients. [Methods] Monoclonal antibody-based dot-ELISA and sandwich ELISA. [Results and Conclusion] The positive rates of dot-ELISA were higher than or similar to those of sandwich ELISA,the former being 48 0%～98 8% and the latter being 12 0%～93 8%. The specificities were 95 4%～100% and 90 0%, respectively.

Key words: schistosomia japonicum, circulating antigen, dot-ELISA, sandwich-ELISA

QIULi-zhuZHANGYong-hongLIHaoXUEHai-chou. COMPARISON OF CIRCULATING ANTIGEN DETECTION IN SCHISTOSOMIASIS JAPONICA PATIENTS USING TWO METHODS OF ELISA [J]. , 2000, 18(4): 10-228.

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[3]	WANGCheng-zu;MOHong-mei;CHENGYu-li;WANGLei;JIANGZi-wei;LIUWen-qi;LIYong-long. Production and Identification of Chicken Egg Yolk Antibodies against Soluble Egg Antigen of Schistosoma japonicum [J]. , 2007, 25(3): 8-197.
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[6]	HUANGHe;YANFuhua;LIChuanjiang;ZHAOMeiyi;TANGJunwen;LIXiangrong. DETECTION OF TOXOPLASMA INFECTION IN WOMEN WITH GYNAECOLOGIC NEOPLASMS USING ELISA  [J]. , 2000, 18(3): 12-166.
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[11]	WUChenyun;LOUWenxian;XUEChunliang. PREPARATION OF MONOCLONAL ANTIBODIES BY INTRASPLENIC IMMUNIZATION OF MICE WITH URINE CIRCULATING ANTIGEN FROM PATIENTS INFECTED WITH SCHISTOSOMA JAPONICUM [J]. , 1999, 17(3): 10-161.
[12]	QIANZhongli;WANGZhaojun;LUPing;G.J.VanDam;A.M.Deelder. PURIFICATION AND SPECIFIC DETECTION OF TWO MAJOR SCHISTOSOMA GUT ASSOCIATED CIRCULATING ANTIGENS, CAA AND CCA * [J]. , 1999, 17(2): 1-69.
[13]	ZhangEnying;WuChenyun;ShenDakang. APPLICATION OF DOT IMMUNOGOLD FILTRATION ASSAY FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA [J]. , 1998, 16(6): 454-456.
[14]	LiuShiguo;ShiYouen;HanJiajun;DengWeiwen. EXPERIMENTAL STUDIES ON DYNAMIC CHANGES OF CIRCULATING ANTIGENS OF SCHISTOSOMAJAPONICUM IN INFECTED RABBITS * [J]. , 1998, 16(4): 283-286.
[15]	QiuLishu;ZhangYonghong;Lihao;XueHaichou;LouWenxian;XuDonghong;QianZhongLi;LuPing;AndrewDeelder. COMPARATIVE OBSERVATION ON THE EFFICACY OF SEVERAL TEST/PROBE SYSTEMS FOR DETECTING SCHISTOSOME CIRCULATING ANTIGEN [J]. , 1998, 16(1): 8-37.

COMPARISON OF CIRCULATING ANTIGEN DETECTION IN SCHISTOSOMIASIS JAPONICA PATIENTS USING TWO METHODS OF ELISA 

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