Histological study of the internal structure of Culicoides arakawaei (Diptera ∶ Ceratopogonidae)

doi:10.12140/j.issn.1000-7423.2021.05.019

Abstract

Abstract:

Objective To study the internal structure of adult Culicoides arakawaei by using the paraffin sectioning technique. Methods C. arakawaei adults were collected, fixed and mounted on slides for morphological identification. The wings and feet of the specimens were detached, and the rest part was processed in Duboscq-Brasil fixative solution for fixation, followed by sequential dehydration, transparenting with xylene, embedding in paraffin, serial sectioning; after stained with hematoxylin-eosin (HE), the midge slices were further dehydrated, transparentized and sealed with neutral gum for morphological observation on the tissue structure by light microscopy. Results Observation on the tissue structure of digestive system, nervous system, respiratory system, and reproductive system of adult C. arakawaei the digestive system is composed of a digestive duct and digestive glands, while the digestive duct comprises foregut, midgut and hindgut, with the midgut being the most developed part; the digestive gland consists of two glands on the left and right sides, including primary and accessory gland. The primary gland functions secretion and the accessory gland deposits the secreta. The central nervous system is composed of the brain and the ventral nerve cord. The brain consists of protocerebrum, deutocerebrum and tritocerebrum. The protocerebrum projects, optic nerve to connect the compound eye; the deutocerebrum extends nerves to the antennae; the periesophageal nerve arises from the tritocerebrum to connect, the subpharyngeal ganglion. The ventral nerve cord contains one subpharyngeal ganglion, three thoracic ganglia and five abdominal ganglia. The respiratory system mainly includes valves and air sacs. The chest of C. arakawaei has two pairs of valves, which are located in the middle and posterior part of chest. The sac mainly includes dorsal and ventral parts, which shaped as tubular or froth form, with weak staining signal. The reproductive system is composed of the female and male internal reproductive organs. The female internal reproductive organs include a pair of ovaries, a pair of oviduct, a spermatheca and a pair of accessory reproductive glands. The male internal reproductive organs include a pair of testes, a pair of spermaduct, an ejaculatory duct and a pair of accessory reproductive glands. Conclusion The internal structure of C. arakawaei is similar to that of other Diptera insects, but significantly different in number, size and morphology of organs in each system.

Key words: Culicoides arakawaei, Adult, Inner structure, Histology

CLC Number:

R384.52

NING Yuan, ZENG Wen-wen, WANG Lin, XU Yuan-yuan, LIU Jiang-xin, DAI Qing-lang, GOUKANG Jin-yuan, LU Xue, HOU Xiao-hui. Histological study of the internal structure of Culicoides arakawaei (Diptera ∶ Ceratopogonidae)[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(5): 696-702.

Figures/Tables 2

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