Laboratory testing and traceability analysis of a case of transfusion-transmitted falciparum malaria in Guangdong Province

Abstract

Abstract:

A clinical case of falciparum malaria with no history of travel but a recent history of blood transfusion, was reported by Guangdong Provincial Center for Disease Control and Prevention in October 2017. We collected clinical data of the patient and carried out an epidemiological traceability survey. Blood samples were collected from the patient and 20 blood donors for malaria rapid diagnostic tests(RDT), microscopic observation of blood smears, and nested PCR. The positive specimens were traced using molecular methods and genotyped. Results of RDT and PCR tests revealed the occurrence of falciparum malaria in the patient and a donor. Their blood samples showed a 100% homology in SSU rRNA sequence, and both were infected with K1 genotype of Plasmodium falciparum. This patient was thus confirmed with Plasmodium falciparum infection via blood transfusion from a donor.

Key words: Malaria, Plasmodium falciparum, Blood transfusion, Transmission, Traceability analysis

CLC Number:

R513.3

Qiang MAO, Fu-quan PEI, Yong-zhen CEN, Meng-ran LIU, Hao ZHANG, Zhuo-hui DENG. Laboratory testing and traceability analysis of a case of transfusion-transmitted falciparum malaria in Guangdong Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(5): 529-533.

Figures/Tables 5

References 21

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引物名称	引物(5′→3′)	长度/bp
属特异性引物	F: CCTGTTGTTGCCTTAAACTTC	1 200
属特异性引物	R: TTAAAATTGTTGCAGTTAAAACG
种特异性引物恶性疟原虫	F: TTAAACTGGTTTGGGAAAACCA- AATATATT	205
种特异性引物恶性疟原虫	R: ACACAATGAACTCAATCATGA- CTACCCGTC
间日疟原虫	F: CGCTTCTAGCTTAATCCACATA- ACTGATAC	120
	R: ACTTCCAAGCCGAAGCAAAGA- AAGTCCTTA
三日疟原虫	F: ATAACATAGTTGTACGTTAAGA- ATAACCGC	141
	R: AAAATTCCCATGCATAAAAAA- TTATACAAA
卵形疟原虫	F: ATCTCTTTTGCTATTTTTTAGTA- TTGGAGA	800
	R: GGAAAGGACACATTAATTGTAT- CCTAGTG

扩增基因	引物	引物(5′→3′)	长度/bp
MSP1	第1轮	F: CTAGAAGCTTTAGAAGATGCAGTATTG
		R: CTTAAATAGATTCTAATTCAAGTGG- ATCA
	第2轮
	K1	F: AAATGAAGAAGAAATTACTACAAAA- GGTGC	150
		R: GCTTGCATCAGCTGGAGGGCTTGCA- CCAGA
	MAD20	F: AAATGAAGGAACAAGTGGAACAGCT- GTTAC	260~390
		R: ATCTGAAGGATTTGTACGTCTTGAAT TACC
	RO33	F: TAAAGGATGGAGCAAATACTCAAGT- TGTTG	270
		R: CAAGTAATTTTGAACTCTATGTTTTA- AATCAGCGTA
MSP2	第1轮	F: GAAGGTAATTAAAACATTGTC
		R: GAGGGATGTTGCTGCTCCACAG
	第2轮
	FC27	F: GCTTATAATATGAGTATAAGGAGAA	330~500
		R: GCATTGCCAGAACTTGAA
	3D7	F: GCTTATAATATGAGTATAAGGAGAA	200~330
		R: CTGAAGAGGTACTGGTAGA