Investigation on species and sporozoite rate of Anopheles spp. in Champasak Province of Laos

Abstract

Abstract:

Objective To investigate the species of Anopheles and rate of Plasmodium sporozoites in anopheline mosquitoes in Champasak Province of Laos, so as to provide essential data for malaria control. Methods Mosquitoes were captured by overnight trapping with light traps and by outdoor human landing catches in Batong County of Champasak Province in July 2017. After morphological identification, genomic DNA was extracted from some female adult mosquitoes, and 18S rRNA-based nested-PCR was performed to detect soprozoites of Plasmodium falciparum and P. vivax. The sporozoite positive rate of Anepheles was calculated. Results A total of 34 687 mosquitoes were captured, belonging to 29 species of 8 genera, dominated by the genus Culex (92.6%, 32 110/34 687), followed by genus Anopheles (5.6%, 1 947/34 687). The genus Anopheles was dominated by An. vagus (65.5%, 1 275/1 947), followed by An. kochi (12.1%, 235/1 947), An. philippinensis (11.9%, 232/1 947), An. sinensis (5.1%, 100/1 947). A total of 336 anopheline female mosquitoes (comprising 234 An. kochi, 100 An. sinensis and 2 An. dirus) were examined by nested-PCR, of which 8 were detected to be positive for P. vivax, including 6 An. kochi (positive rate 2.6%, 6/234) and 2 An. sinensis (2.0%, 2/100). No soprozoites of P. falciparum were found. Nested-PCR amplification showed a band of ~120 bp, sequence of which had 99%-100% homology to those of P. vivax (GenBank Accession No: KT991325, KT991317, MG708221, MF540772, LT635613, KU569498, AF145335, KT991314 and KX007932). Conclusion Both An. kochi and An. sinensis mosquitoes carry P. vivax sporozoites with a positive rate of 2.6% and 2.0% respectively.

Key words: Malaria, Anopheles, Sporozoite, Nested-PCR, Laos, Champasak Province

CLC Number:

R384.11

Wei-li WANG, Rui YANG, Chun-hai LUO, Xiao-fang GUO, Jin-yong JIANG, Hong-ning ZHOU, SORCHAMPA Somphad, VANNAPASEUTH Phoxay, Cang-lin ZHANG. Investigation on species and sporozoite rate of Anopheles spp. in Champasak Province of Laos[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2018, 36(5): 478-482.

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References 37

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