Evaluation of the testing ability of city/prefecture-level malaria laboratories in Guizhou Province during 2014-2016

Abstract

Abstract:

Objective To re-examine the blood smears and the dry blood filter papers submitted by the city/prefecture-level malaria laboratories in the Malaria Reference Laboratory of Guizhou Province, to evaluate the laboratory testing ability of the city/prefecture-level malaria laboratories. Methods Microscopy and nested PCR were performed to re-examine the blood smears and dry blood filter papers in the Reference Laboratory during 2014-2016, and the blood smear quality was evaluated. Using the microscopic results of the Reference Laboratory as a standard, the malaria diagnostic coincidence concerning positive infection rate, negative rate and species identification were determined. The microscopic and nested PCR results of the Reference Laboratory were analyzed as well.Results From 2014 to 2016, a total of 163 blood smear samples, 84 positive and 79 negative tested in city/prefecture-laboratories, and 159 dry blood filter papers were submitted, with a submission rate of 100% (163/163) and 97.6% (159/163), respectively. The qualification rates of blood smear preparation, staining, cleanliness and comprehensive judgment were 58.9% （96/163）, 75.5% （123/163）, 72.4% （118/163）, and 69.9% (114/163), respect-ively. The qualification rates of blood smear preparation （71.3%） and comprehensive judgment （95.0%） in 2016 were both higher than those in 2014（46.4%,46.4%） and 2015（48.1%, 44.4%）（P < 0.05). The total coincidence rates of microscopic results of the city/prefecture-level malaria laboratories during 2014-2016 were 67.9% （38/56）, 66.7% （18/27）, and 88.8% (71/80), respectively, being highest in 2016（P < 0.05). The coincidence rates of positive infection during 2014-2016 were 81.3% (26/32), 70.0% (14/20) and 96.9% (31/32), respectively, with no significant difference among the three years (P > 0.05). The coincidence rates of species identification during 2014-2016 were 46.9% （15/32）, 65.0% （13/20） and 81.3% （26/32）, respectively （2014 vs. 2016, P < 0.05）. The coincidence rates of negative infection during 2014-2016 were 95.8% (23/24), 5/7 and 93.8% （45/48）, respectively, with no significant difference among the three years（P > 0.05). The consistency rate between microscopic and nested PCR results in the Reference Laboratory was 44.0% （70/159） with respect to positive results and species identification, and 48.43% （77/159) with respect to negative results, and the inconsistency rate was 7.6% （12/159）. Conclusion The qualification rate of comprehensive judgment and the total coincidence rate of microscopic results in the city/prefecture-level malaria laboratories was higher in 2016 than in 2014 and 2015. The results suggested that the testing ability of city/prefecture-level malaria laboratories has increased.

Key words: Malaria, Laboratory, Microscopy, Nested PCR

CLC Number:

R382.31

Dan-ya SHE, Jia-hong WU, Li-dan LU, Yu-ting HUANG, Yan GENG, Nian-heng ZHANG. Evaluation of the testing ability of city/prefecture-level malaria laboratories in Guizhou Province during 2014-2016[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(5): 466-471.

Figures/Tables 4

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Table 4

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疟原虫种类 Plasmodium species	引物名称 Primer name	引物序列 Primer sequence	产物长度/bp Product size/bp
疟原虫Plasmodium spp.	rPLU5	5′-CCTGTTGTTGCCTTAAACTTC-3′	1 200
疟原虫Plasmodium spp.	rPLU6	5′-TTAAAATTGTTGCAGTTAAAACG-3′
恶性疟原虫P. falciparum	rFAL1	5′-TTAAACTGGTTTGGGAAAACCAAATATATT-3′	205
恶性疟原虫P. falciparum	rFAL2	5′-ACACAATGAACTCAATCATGACTACCCGTC-3′
间日疟原虫 P. vivax	rVIV1	5′-CGCTTCTAGCTTAATCCACATAACTGATAC-3′	120
间日疟原虫 P. vivax	rVIV2	5′-ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA-3′
三日疟原虫 P. malariae	rMAL1	5′-ATAACATAGTTGTACGTTAAGAATAACCGC-3′	141
三日疟原虫 P. malariae	rMAL2	5′-AAAATTCCCATGCATAAAAAATTATACAAA-3′
卵形疟原虫P. ovale	rOVA1	5′-ATCTCTTTTGCTATTTTTTAGTATTGGAGA-3′	800
卵形疟原虫P. ovale	rOVA2	5′-GGAAAGGACACATTAATTGTATCCTAGTG-3′

方法 Methods		镜检 Microscopy					合计 Total
						阴性数 No. negative
		恶性疟原虫 P. falciparum	间日疟原虫 P. vivax	三日疟原虫 P. malariae	卵形疟原虫 P. ovale	阴性数 No. negative
巢式PCR Nested PCR	恶性疟原虫P. falciparum	36	0	0	0	7	43
	间日疟原虫P. vivax	0	24	0	2	0	26
	三日疟原虫P. malariae	0	0	2	0	0	2
	卵形疟原虫P. ovale	0	0	0	8	0	8
	混合感染^a Mixed infection^a	1	0	0	2	0	3
	阴性数No. negative	0	0	0	0	77	77
合计 Total		37	24	2	12	84	159

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