Differential analysis of transcriptomes in Boettcherisca peregrine pupae at three developmental stages

Abstract

Abstract: Objective To investigate the differential expression of transcriptomes in Boettcherisca peregrine pupae at three developmental stages. Methods Total RNA was extracted from B. peregrine pupae at stages of day 1(MY1), day 5(MY2) and day 10(MY3), reverse-transcribed into cDNA, and sequenced. Gene differential expression was analyzed with edgeR software. GO enrichment analysis was made for genes with differential expression. Analysis of KEGG(Kyoto Encyclopedia of Genes and Genomes) metabolic pathway was made for Universal Gene (Unigene). The simple sequence repeat(SSR) of Unigene was detected with MISA. Results A total of 38 727 Unigenes were obtained after differential expression analysis on cDNA at the three stages. The numbers of genes with differential expression between each two stages were 1 263-2 430, with an overall number of 5 283. Pattern clustering analysis revealed similar expression patterns of those genes at the three stages. A total of 8 856 Unigenes and 336 pathways were annotated for B. peregrine pupae. A total of 12 146 SSR were detected from 8 645 Unigenes(22.3%), comprising predominantly of single-nucleotide repeats(69.23%), then the three-nucleotide repeat(21.0%). Of the SSR, the A/T occurred most frequently, followed by AAC/GTT and AG/GT. Conclusions There are significant differences in gene expression in B. peregrine pupae at different developmental stages.

Key words: Boettcherisca peregrine, Pupa, Transcriptome, Differential expression of genes

CLC Number:

R384.21

Hao ZHOU, Qi-yan WANG, Hong-ling ZHANG, Zheng REN, Jia-lin DAI, Yu-bo LIU, Jie WANG, Jiang HUANG. Differential analysis of transcriptomes in Boettcherisca peregrine pupae at three developmental stages[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(3): 274-279.

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基因名称 Gene name	引物名 Primer name	引物序列（5′→ 3′） Primer sequences（5′→ 3′）	长度/bp Length/bp	退火温度/℃ Tm/℃
TH	TH-F： TH-R：	ATGGTGCTGGTCTCTTGTCC ATACCCAACGACGGAACTTG	3 625	60
CBM-14	CBM-14-F： CBM-14-R：	AAACACCACGGAAATCATCC CCAGAAGCAATCATCCCAAT	663	60
Tubulin	Tubulin-F： Tubulin-R：	GCTTCCTCATCTTCCACTCGTTC GATGTCGTAGATAGCCTCGTTGTC	1 353	60

代谢通路ID Metabolic pathway ID	代谢通路 Metabolic pathway	被注释的数量 Annotated number	所占比例/% Percentage/%
ko01100	生化代谢Metabolic pathways	1 353	15.28
ko01110	次生代谢产物的生物合成Biosynthesis of secondary metabolites	438	4.95
ko05016	亨廷顿氏舞蹈症Huntington's disease	405	4.57
ko01120	微生物的代谢Microbial metabolism in	273	3.08
ko05010	阿尔茨海默病Alzheimer's disease	254	2.87
ko05200	恶性肿瘤通路Alzheimer's disease	229	2.59
ko00230	嘌呤代谢Purine metabolism	214	2.42
ko00190	氧化磷酸化Oxidative phosphorylation	212	2.39
ko05012	帕金森病Parkinson's disease	199	2.25
ko05166	HTLV-I感染HTLV-I infection	198	2.24

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