Hepatic pathological changes in buffaloes infected with Fasciola gigantica

Abstract

Abstract: Objective To observe the hepatic pathological changes in buffaloes at different times after infection with Fasciola gigantica metacercariae. Methods Twenty-nine healthy marshy water buffaloes were divided into infection group (n = 24) and control group (n = 5). Buffaloes in the infection group were orally given 500 metacercariae and sacrificed at day 3, 10, 28, 42, 70 (n = 5, 5, 5, 4, 5) post infection. The control group received no treatment. The hepatic pathological changes were examined by visual observation and hematoxylin-eosin (HE) staining on liver tissue sections. Masson’s trichrome stain was performed to visualize collagen protein in liver tissue. Results A gray-white tablet-shaped lesion was seen scattering on liver surface at day 3 post infection, which became more significant at day 10, accompanied by the formulation of scar. Purulent nodules began to appear on the liver surface from day 28 and increased at day 42 and day 70. The control group showed a smooth liver surface with no pathological signs. HE staining showed structural disorder of hepatic cord and granular degeneration of hepatic cells at day 3, karyolysis and karyorrhexis at day 10, eosinophil infiltration and localized hemorrhage in hepatic lobules at day 28, massive hemorrhage in hepatic lobules at day 42, as well as fibrosis between sinusoids and hepatic cell repair at day 70. The control group showed a clear structure of hepatic cord and intact hepatic cells. The Masson’s trichrome stain showed formation of a small amount of collagen fibers in the portal areas and sinusoids at day 42, which increased at day 70, while the control group had only a slight sign of collagen fibers. Conclusion There occur dynamic hepatic pathological changes in buffaloes at different times after F. gigantica infection. The hepatic lesions begin to appear at as early as at day 3, then become more serious with time, accompanied with inflammatory reactions, until fibrosis, a typical sign of hepatic pathology, at day 42.

Key words: Fasciola gigantica, Bufflao, Liver, Pathological change

CLC Number:

R383.26

Zhi-yong WEI, Zhao-an SHENG, Yi-ying LIANG, Kai ZHANG, Wei-yi HUANG. Hepatic pathological changes in buffaloes infected with Fasciola gigantica[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(3): 254-258.

Figures/Tables 3

References 19

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