Polymorphisms of tryptophan-rich antigen gene of Plasmodium ovale subspecies from imported malaria cases in Henan Province

Abstract

Abstract:

Objective To analyze the genetic sequence of tryptophan-rich antigen (PoTRA) gene of Plasmodium ovale subspecies from imported malaria cases in Henan Province in 2015. Methods Blood samples were collected from 22 imported ovale malaria cases in Henan Province in 2015. After DNA extraction, PoTRA was amplified by nested PCR, and was inserted into the pMD18-T vector. The plasmid was extracted and sequenced, and the results were blasted in GenBank to determine the subspecies of P. ovale. The sizes and species of the PoTRA gene were analyzed. The amino-acid sequence of PoTRA was also aligned to analyze the difference in amino acid sequence. The phylogenetic tree was constructed to analyze the genetic relationship among the samples by neighbor-joining. Results Of the 22 imported cases, eight (36.4%) were infected with P. ovale wallikeri, which had two sizes, the predominant 245 and 299 bp. The other 14 cases (63.6%) were infected with P. ovale curtisi, which had three sizes, the predominant 299, 317 and 335 bp. Amino-acid sequence alignment revealed that the two types of P. ovale wallikeri differed in two amino-acid units, MANPINMANPIN and AITPIN, while the three types of P. ovale curtisi differed in amino-acid units TITPIS and TINPIN. The phylogenetic tree showed that the 22 samples belonged to two subpopulations of P. ovale curtisi and P. ovale wallikeri, wherein the P. ovale curtisi was further divided into two sub-branches, and samples with sizes of 317 and 335 bp were in the same sub-branch with a closer genetic relationship. Conclusion Two subspecies, P. ovale curtisi and P. ovale wallikeri, are identified from the imported ovale malaria cases in Henan Province in 2015. The P. ovale curtisi has three genetic types and P. ovale wallikeri has two genetic types of PoTRA gene, revealing genetic polymorphisms of PoTRA.

Key words: Imported ovale malaria, P. ovale curtisi, P. ovale wallikeri, Tryptophan-rich antigen

CLC Number:

R382.319

Rui-min ZHOU, Su-hua LI, Ya-lan ZHANG, Dan QIAN, Cheng-yun YANG, Ying LIU, Yu-ling ZHAO, Hong-wei ZHANG, Bian-li XU. Polymorphisms of tryptophan-rich antigen gene of Plasmodium ovale subspecies from imported malaria cases in Henan Province[J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(1): 13-17.

Figures/Tables 3

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样本编号 Sample code	国家 Country	卵形疟亚种 P. ovale subspecies	PoTRA长度/bp Size of PoTRA/bp
Po01	安哥拉	wallikeri	245
Po02	安哥拉	wallikeri	245
Po03	安哥拉	wallikeri	245
Po04	喀麦隆	wallikeri	245
Po05	喀麦隆	wallikeri	245
Po06	刚果	wallikeri	299
Po07	尼日利亚	wallikeri	299
Po08	尼日利亚	wallikeri	245
Po09	安哥拉	curtisi	299
Po10	尼日利亚	curtisi	299
Po11	尼日利亚	curtisi	299
Po12	赤道几内亚	curtisi	299
Po13	赤道几内亚	curtisi	299
Po14	加纳	curtisi	299
Po15	加纳	curtisi	299
Po16	利比里亚	curtisi	299
Po17	坦桑尼亚	curtisi	299
Po18	安哥拉	curtisi	317
Po19	尼日利亚	curtisi	317
Po20	刚果	curtisi	317
Po21	几内亚	curtisi	317
Po22	喀麦隆	curtisi	335