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The Application of Gold-immunochromatographic Test Strip Reader in Serum Antibody Detection in Echinococcosis

XIE Gui-lin1 , YIN Jun-xia2 , DUAN Xin-yu3 , FENG Xiao-hui3   

  1. 1 Department of Hepatobiliary Surgery,the Second Hospital of Shaoxing City, Shaoxing 312000, China; 2 Health and Family Planning Commission of Tianmen City of Hubei Province, Tianmen 431700, China; 3 Xinjiang Hydatid Clinical Research Institute, Urumqi 830011, China; 4 College of Biomedical Engineering & Instrument Science, Zhejiang University, Huangzhou 310013, China; 5 College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250014, China
  • Online:2015-06-30 Published:2015-09-08

Abstract:

【Abstract】 One hundred and fifty-nine serum samples from hydatid disease patients and 80 serum samples from patients with other liver diseases were detected by gold-immunochromatographic assay, and read by naked eyes and the gold-immunochromatographic test strip reader. The sensitivity, specificity, and accuracy of eye-based method was 92.4% (147/159), 85.0% (68/80), and 89.9% (215/239), which was lower than that of the reader detection (95.6%, 93.7%, 95.0%, respectively). While, its false negative rate (7.5%, 12/159) and false positive rate (15.0%, 12/80) was higher than that of the reader detection (4.4% and 6.3%, respectively).

Key words: Gold-immunochromatographic assay, Quantitative detection, Gold-immunochromatographic test strip reader, Hydatid disease, Antibody