An Observation on the Histological Structure of Oncomelania hupensis Soft Tissue by Agar-Paraffin Double-embedding Method

Previous Articles Next Articles

An Observation on the Histological Structure of Oncomelania hupensis Soft Tissue by Agar-Paraffin Double-embedding Method

TAN Ping*，ZHANG Jie，LI Qing，YU Zhi-jun

Department of Parasitology，Medical College of Wuhan Science and Technical University，Wuhan 430065，China

Online:2014-12-30 Published:2015-02-12

Abstract

Abstract:

In order to study the histological structure of Oncomelania hupensis soft tissue， the fixed soft tissues of O. hupensis were pre-embedded in the agar and made blocks， then dehydrated， transparentized， immersed in paraffin， sectioned， and stained with haematoxylin-eosin（HE）. Permanent slides of O. hupensis soft tissue were obtained. The histological structure of soft tissues was clear under the microscope.

Key words: Oncomelania hupensis, Soft tissue, Agar-Paraffin double-embedded

TAN Ping*，ZHANG Jie，LI Qing，YU Zhi-jun. An Observation on the Histological Structure of Oncomelania hupensis Soft Tissue by Agar-Paraffin Double-embedding Method[J]. .

[1]	CUI Guo-yan 1,2, WANG Shi-ping, CHENG Hong-bing, WEI Hong, HE Xin, LVQIU Si-jia, HUANG Cheng-ming. Screening of Molluscacidal Microorganisms against Oncomelania hupensis* and Their Effect [J]. , 2015, 33(3): 17-234-236.
[2]	GUO Dan-Zhao, CHEN Jun. Biological Safety of the Molluscicidal Ingredient from Aspergillus fumigatus SL-30 Isolated from Rhizosphere of Phytolacca acinosa [J]. , 2012, 30(6): 10-460-463.
[3]	WANG Ke-geng1，ZENG Qing-ren2，ZHANG Yu-kuai1，ZHOU Jun3，CAI Li-ting，LIANG Yu1，LIU Yan1,2 *. Preparation of Agar-Paraffin Double-Embedded Longitudinal Sections of Schistosoma japonicum [J]. , 2012, 30(5): 20-415-417.
[4]	MA Xian-liang，LIU Qin，ZHANG Yi. Cloning, Expression and Activity Analysis of Full-length Gene Encoding Thioredoxin Peroxidase from Oncomelania hupensis [J]. , 2012, 30(4): 3-262-267.
[5]	LI Shi-zhu，ZHANG Li，LIU Qin，LV Shan，WANG Qiang，QIAN Ying-jun，YANG Kun，ZHOU Xiao-nong. Study on the Genetic Differences among Oncomelania hupensis Population in Middle and Lower Reaches of Yangtze River using Microsatellite DNA Markers [J]. , 2012, 30(4): 4-268-273.
[6]	ZHOU Shu-Lin, PENG Huai-ming, LI Chao-Pin, LIU Hui, ZHAO Jin-song, CAO Zhi-Guo. Isolation and Purification of Oncomelania hupensis Agglutinin and Determination of its Molecular Weight [J]. , 2011, 29(6): 11-453-457.
[7]	PENG Huai-ming1,2，LI Chao-pin1，LIU Hui1，ZHAO Jin-song1，ZHOU Shu-lin1 . Extraction of Agglutinin from Oncomelania hupensis* and its Haemagglutination Activity [J]. , 2011, 29(2): 10-130-133.
[8]	MAAn-ning;NIHong;WANGWan-xian;ZHANGYun;GENGPeng. Luring Effect of the Fermented Laminaria japonica*to Oncomelania hupensis [J]. , 2010, 28(1): 12-53.
[9]	ZHOUXiao-nong;LIShi-zhu;LIUQin;ZHANGYi. Advances on Genetics and Biological Characteristics of Oncomelania hupensis [J]. , 2009, 27(5): 7-418.
[10]	YANGXun;CHENJun;HANBang-xing;DAIJian-ping;BEIYu-fei;HANZhi-yi. Molluscicidal Activity of Endophyte JJ18 from Pseudolarix kaempferi* Inoculated to Beach Soil [J]. , 2009, 27(4): 21-372.
[11]	NIHong;MAAn-ning;ZHANGYun;GENGPeng. Preliminary Study on Molluscicidal Effect of Active Components from Centipeda minima [J]. , 2009, 27(4): 24-378.
[12]	WUYue-ying;NINGAn;ZHOUXiao-hong;XUJing;LIJian-ren;ZHENGShui-fang;CHENGXiao-bo. Molluscicidal Effect and Toxicity of META-Li on Oncomelania* Snails in the Mountain Area [J]. , 2009, 27(3): 24-287.
[13]	TANPing;ZHANGXue-jun;YANGJian-ming;ZHANGYan. Effect of Alternanthera philoxeroides* on Enzymic Histochemistry of Oncomelania hupensis [J]. , 2009, 27(1): 3-16.
[14]	HUYing;LIXue-ming;LINRui;NIUAn-ou;HUWen-qing. Genetic Variation of Two Mitochondrial DNA Molecules from Three Isolates of Oncomelania hupensis [J]. , 2007, 25(6): 9-477.
[15]	YEQing;ZHUJun-yong;ZHONGQin-ping;JIANGMing-sen;DONGHui-fen*. Primary Culture of the Cells from Oncomelania hupensis Liver [J]. , 2007, 25(6): 10-482.

An Observation on the Histological Structure of Oncomelania hupensis Soft Tissue by Agar-Paraffin Double-embedding Method

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments