Abstract: The amino acid sequences of β-tubulin from Toxoplasma gondii stains （GT1 and ME49） and human were aligned by ClustalW2 software. Based on the alignment result， the C-terminal peptides of β-tubulin of T. gondii were artificially synthesized. Rabbits were immunized with 0.5 mg synthesized peptides for five times at 2-week intervals. Serum samples were collected at the second week after the final immunization， and were analyzed for specific antibodies by ELISA. Finally， the specific-β-tubulin polyclonal antibody was evaluated by Western blotting with the total protein of RH strain， ME49 strain， and PRU strain of T. gondii， respectively. The results showed that β-tubulin of T. gondii stains（GT1 and ME49） shared 100% amino-acid sequence identity， and there was 98% amino acid homology between T. gondii and human. The main variable region was the C-terminus. After the fifth immunization， the titers of polyclonal antibody reached 1 ∶ 52 800. Western blotting result indicated that the specific-β-tubulin polyclonal antibody reacted with β-tubulin in all the three strains （RH， ME49， and PRU）， respectively.

Key words: Toxoplasma gondii；&beta, -tubulin；Polyclone antibody