Construction and Identification of the Bifidobacterium Expression System pGEX-TSOL18/B. longum of Taenia solium

›› 2014, Vol. 32 ›› Issue (3): 18-239-241.

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Construction and Identification of the Bifidobacterium Expression System pGEX-TSOL18/B. longum of Taenia solium

ZHOU Bi-ying*，LIU Mei-chen，HE Li-fang

Department of Parasitology，Zunyi Medical College，Zunyi 563003，China

Online:2014-06-30 Published:2014-09-17

Abstract

Abstract: The TSOL18 gene of Taenia solium was synthesized and cloned into Escherichia coli-Bifidobacteria shuttle vector pGEX-1λT. The recombinant plasmid pGEX-TSOL18 was transformed into Bifidobacterium longum with electroporation. The recombinant plasmid containing TSOL18 gene was identified by restriction endonuclease analysis， PCR and DNA sequencing. The length of synthesized TSOL18 gene was 393 bp. The results indicated that the Bifidobacteria expression system pGEX-TSOL18/B. longum was successfully constructed.

Key words: Taenia solium, Bifidobacterium, TSOL18, Recombinant bacterium

ZHOU Bi-Ying, LIU Mei-Chen, HE Li-Fang. Construction and Identification of the Bifidobacterium Expression System pGEX-TSOL18/B. longum of Taenia solium[J]. , 2014, 32(3): 18-239-241.

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Construction and Identification of the Bifidobacterium Expression System pGEX-TSOL18/B. longum of Taenia solium

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