Abstract: A total of 2 769 children with allergic disease were admitted from October 2010 to December in Ningbo Women & Children′s Hospital. Fourteen kinds of serum specific-IgE were detected by immunoblotting method. Among the 2 769 children, the total positive rate of sIgE was 76.0%（2 105/2 769）. The sIgE positive rate in males （80.0%, 1 344/1 679） was significantly higher than that of females（69.8%, 761/1 090）（P<0.05）. The top three allergens were dust mites（33.2%）, milk（12.6%） and fungi（12.6%）. There were statistical differences of positive reaction to dust mites among 4 seasons（19.4%, 33.9%, 39.3%, 36.0%）（P<0.05）. Significant differences were found in the positive rates of dust mites, milk, fungi, and egg among the age groups（P<0.05）. The level of dust mite specific IgE were mainly in grade 3-6（613/918, 66.8%）.

Key words:  , Children；Allergen detection；IgE；Immunoblotting