›› 2012, Vol. 30 ›› Issue (6): 20-498-499.

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A Single Staining Method for Specimen of Trichinella spiralis Encapsulated Larvae

YANG Ding1,PI Ben-wei1,NIU Li-na1,LI Dan1,ZHANG Ying1,WANG Guo-ying2 *   

  1. 1 Clinical Medicine,Medical College,Henan University,Kaifeng 475000,China;2 Laboratory of Cellular and Molecular Immunology,Medical College,Henan University,Kaifeng 475004,China
  • Online:2012-12-31 Published:2013-02-05

Abstract: Trichinella spiralis encapsulated larvae speciemens were stained with acetate alum carmine staining solution. Four procedures were compared: ① speciemen stained for 20 h, and faded; ② speciemen stained for 2.5 h without fading; ③ speciemen stained for 20 h without fading; ④ speciemen stained for 20 h, and faded. The results showed that the first method is optimal, which clearly distinguishes the capsula, encapsulated larva and muscle cells.

Key words: Trichinella spiralis, Encapsulated larvae, Staining