›› 2011, Vol. 29 ›› Issue (1): 16-67-70.

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Cloning and Sequence Analysis of Thioredoxin Peroxidase Gene from Taenia multiceps

LI  Yong-Guang-1, 2 , LI  Wen-Hui-1, GAI  Wen-Yan-1, TAO  Ju-Xia-1, QU  Zi-Gang-1, GU  Mo-Zhong-1, Radu  Blaga3, FU  Bao-Quan-1 *   

  1. 1 Key Laboratory of Animal Parasitology of Gansu Province；Key Laboratory of Veterinary Public Health of the Ministry of Agriculture；State Key Laboratory of Veterinary Etiological Biology；Lanzhou Veterinary Research Institute，Chinese Academy of Agricultural Sciences，Lanzhou 730046，China；2 College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070，China；3 Faculty of Veterinary Medicine，University of Agricultural Sciences and Veterinary Medicine，Cluj-Napoca 400372，Romania
  • Online:2011-02-28 Published:2012-09-27

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Abstract: Protoscoleces of Taenia multiceps were collected from the naturally infected sheep and total RNA was extracted. Specific primers were designed according to TaHc2-D11 mRNA sequence and T. multiceps thioredoxin peroxidase gene （TmTPx） was amplified by RT-PCR. PCR products were ligated into pMD18-T vector and transformed to E. coli DH5α. The recombinant plasmids were identified by restriction digestion and sequencing. A 614 bp cDNA was amplified. The TmTPx open reading frame （591 bp） encoded a 196-amino acid protein with Mr 21 690, pI 7.61. Bioinformatics analysis indicated that TmTPx had a typical 2-Cys Prx conserved domain. Phylogenetic tree revealed that T. multiceps had the closest relationship to T. asiatica， followed by T. solium and T. crassiceps， E. granulosus and E. multilocularis.