Abstract: Objective To investigate the species of blood-sucking midges of Shanghai. Methods The specimens were collected by light-trap from Pudong Airport （reed marshes）, Shanghai， during the years of 2007-2009. The captured samples was briefly classified, and mounted slide specimens with phenol-balsam method. The microscopic examination including morphological and numerical characters for species identification. Results A new species of biting midges named Culicoides （Beltranmyia） shanghaiensis sp. nov. was obtained， its diagnostic characters were as follows: ① wing without distinct pale or dark markings， only 0-7 macrotrichia find in basal cell; ② the female eyes separate， with pubescence between facets， proboscis head ratio （P/H） 0.7, while the head proboscis ratio （H/P） 1.45, antennal ratio （AR） 1.18, sensila coeloconica present on segments 3-14 （frequency: 1.0, 0.7, 0.5, 0.5, 0.7, 0.5, 0.8, 0.7, 1.0, 1.0, 1.0, 1.0, respectively, n=17） and absent on segments 4-10 indefinitely， with one developed spermatheca；③ the male′s parameres fused narrowly on base. The new species is allied to Culicoides homochrous Remm，but can be distinguished chiefly by: its wing with numerous macrotrichias distributing in basal cell; female eyes separate, bare, H/P 1.03-1.04; male′s parameres separate. Besides, the Culicoides charadraeus Arnaud and Culicoides rarus Das Gupta also resembling with the new species, it can be differentiated from the two former species by the female eyes bare and contiguous （or narrowly separated）, AR＞1.61, no macrotrichia in basal cell of the wings, and the different male genitalia features. Conclusion A new species of biting midges collected from Shanghai is described, and some diagnostic characters are discussed for distinguishing the closely allied species.

Key words: Diptera, Ceratopogonidae, Culicoides shanghaiensis, New species