Establishment of Loop-mediated Isothermal Amplification （LAMP） for Detecting Pneumocystis carinii

›› 2010, Vol. 28 ›› Issue (4): 18-307.

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Establishment of Loop-mediated Isothermal Amplification （LAMP） for Detecting Pneumocystis carinii

ZHANG Hui，LIU Xue-qing，HE Rong-zhi，JIA Tian-jun，ZHANG Jin-shun*

Institute of Pathogenic Biology and Immunology，Hebei North University，Zhangjiakou 075000，China

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-30 Published:2010-08-30

Abstract

Abstract: Animal model of Pneumocystis carinii pneumonia （PCP） was established for acquiring lung tissue infected with P. carinii. After DNA from rat lungs was extracted， nuclear ribosome small subumit 18s rDNA of P. carinii was amplified by loop-mediated isothermal amplification method at 63 ℃ for 60 min. The product was digested by restriction enzyme ApalⅠ. The results showed that 18s ribosome DNA （rDNA） of P. carinii was cloned into vector pGEX6p2， and the positive clones screened. Therefore, the loop-mediated isothermal amplification has been established for detecting P. carinii.

Key words: Pneumocystis carinii, 18s rDNA, Loop-mediated isothermal amplification

ZHANGHui;LIUXue-qing;HERong-zhi;JIATian-jun;ZHANGJin-shun*. Establishment of Loop-mediated Isothermal Amplification （LAMP） for Detecting Pneumocystis carinii[J]. , 2010, 28(4): 18-307.

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Establishment of Loop-mediated Isothermal Amplification （LAMP） for Detecting Pneumocystis carinii

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