Establishment of Loop-mediated Isothermal Amplification （LAMP） for Detecting Pneumocystis carinii

›› 2010, Vol. 28 ›› Issue (4): 18-307.

• 研究简报 • Previous Articles Next Articles

Establishment of Loop-mediated Isothermal Amplification （LAMP） for Detecting Pneumocystis carinii

ZHANG Hui，LIU Xue-qing，HE Rong-zhi，JIA Tian-jun，ZHANG Jin-shun*

Institute of Pathogenic Biology and Immunology，Hebei North University，Zhangjiakou 075000，China

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-30 Published:2010-08-30

Abstract

Abstract: Animal model of Pneumocystis carinii pneumonia （PCP） was established for acquiring lung tissue infected with P. carinii. After DNA from rat lungs was extracted， nuclear ribosome small subumit 18s rDNA of P. carinii was amplified by loop-mediated isothermal amplification method at 63 ℃ for 60 min. The product was digested by restriction enzyme ApalⅠ. The results showed that 18s ribosome DNA （rDNA） of P. carinii was cloned into vector pGEX6p2， and the positive clones screened. Therefore, the loop-mediated isothermal amplification has been established for detecting P. carinii.

Key words: Pneumocystis carinii, 18s rDNA, Loop-mediated isothermal amplification

ZHANGHui;LIUXue-qing;HERong-zhi;JIATian-jun;ZHANGJin-shun*. Establishment of Loop-mediated Isothermal Amplification （LAMP） for Detecting Pneumocystis carinii[J]. , 2010, 28(4): 18-307.

[1]	LIN Weina, DENG Wangping, YANG Ying, LI Yinlong, QIN Zhiqiang, HONG Yang, FENG Ting, LV Chao, XU Jing. Establishment and preliminary evaluation of a rapid assay for detection of Schistosoma japonicum nucleic acid based on LAMP-CRISPR/Cas12a [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2025, 43(2): 167-174.
[2]	JIA Xishuai, ZHOU Shuimao, LUO Huatang, LIU Cong, WANG Shuai, XU Wenxiu. Establishment and application of loop-mediated isothermal amplification for detection of Leishmania [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(3): 413-417.
[3]	JIANG Ning, BAI Jie, LI Ping, SHAN Wenqi, ZHOU Qiuming, DONG Haowei, YUAN Hao, TAO Feng, LI Xiangyu, MA Yajun, PENG Heng. Establishment of a rapid detection method of mosquito-borne dengue virus based on loop-mediated isothermal amplification microfluidic chip technology [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2024, 42(2): 234-241.
[4]	CHEN Huilin, ZHU Yonghang, FAN Haiye, ZHAO Wei, LIU Luyao, CAO Yuxiang, YE Changjiang, SUN Entao. Comparative study on DNA barcoding of house oribatid mites based on the 18S and 28S ribosomal DNA gene [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2023, 41(2): 163-169.
[5]	JIANG Feng, CHEN Run, DU Ning-ning, ZHU Meng-yi, ZHONG Hao, CHEN Hui, XI Xu-xia, ZHAN Xiao-dong, LI Chao-pin. Investigation of Toxoplasma gondii infection in pet dogs and cats in Wuhu City [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2022, 40(1): 124-126.
[6]	CHEN Mu-xin1,2, CHEN Jia-xu2, LIU Wei2, FENG Xin-yu2, CHEN Shen-bo1,2,CHEN Shao-hong2, CAI Yu-chun1,2, XU Bin2, HU Wei1,2*. Sequencing and Phylogenetic Tree Construction of 18S Ribosomal DNA from Five Species of Plasmodium from Yunnan Border between China and Myanmar and Other Areas [J]. , 2016, 34(6): 4-493-499.
[7]	GAO Shan-shan1,2, WU Shao-qiang3, LUO Jing1, WANG Cheng-min1, ZHANG Min1,. Development of an IMS-qPCR Method for Detection of Cryptosporidium parvum in Water [J]. , 2013, 31(3): 4-180-184.
[8]	CHENJin-ling;DUANYi-nong;WANGJian-xin;ZHUDan-dan;QINYong-wei. Immunogenicity of p55 Gene Fragment from Pneumocystis carinii* [J]. , 2009, 27(6): 9-497.
[9]	ZHOULing;ZHOUBi-ying*. Influence of Baicalin on TNF-α and Soluble Intercellular Adhesion Molecule-1 in Rats Infected with Pneumocystis carinii [J]. , 2009, 27(2): 11-147.
[10]	YANGQiu-lin*;ZHANGRu-sheng;WUHe-ping;ZHANGYu-kuai;WANGKe-geng. Detection of Toxoplasma gondii DNA by Loop-Mediated Isothermal Amplification [J]. , 2008, 26(4): 14-306.
[11]	DAIXiao-dong;QINYuan-hua;ZHOUChang-hai;ZHENGLi-li;SHAOWei-jun;TAOLin;CUIYu. Effects of Fructus psoraleae and Brucea javanica on the Level ofIL-2 and NK cell in Rats Infected with Pneumocystis carinii [J]. , 2007, 25(5): 21-438.
[12]	WANGJian-xin;;DUANYi-nong;CHENJin-ling;DONGYong-sheng. Construction and Expression of Prokaryotic Expression Plasmids of Pneumocystis carinii p55 Antigen Gene Fragment [J]. , 2007, 25(4): 13-332.
[13]	LUZhi-min;ZHANGYou-cheng;ZHANGJin-shun;SHIBao-lin;JIATian-jun;TANGHong-wei;LIUJin-hua. Enzyme Change in Bronchoalveolar Lavage Fluid of Pneumocystis Pneumonia Rats and the Effect of Garlicin Treatment [J]. , 2007, 25(4): 7-303.
[14]	HUANGMin-jun;ANYi-jun;LIShu-zhen;LUSi-qi;GUOZeng-zhu. Continuous Axenic Cultivation of Pneumocystis carinii Isolated from the Bronchoalveolar Lavage Fluid of Infected Rat [J]. , 2007, 25(2): 11-132.
[15]	LIUKe-qin;YINWei-dong;XUEGui-ping;ZHANGJin-shun. Study on the Changes of Soluble Intercellular Adhesion Molecule-1 Level in Sera of Rats Infected with Pneumocystis carinii [J]. , 2007, 25(1): 6-31.

Establishment of Loop-mediated Isothermal Amplification （LAMP） for Detecting Pneumocystis carinii

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments