Abstract: Volatile oil from the clove bud was extracted by petroleum ether using Soxhlet Extractor. The acaricidal activity was examined using direct contact and vapour phase toxicity bioassays. In a filter paper contact toxicity bio-assay, at 2.5 h after treatment, clove bud oil at a dose of 12.20 μg/cm² killed all dust mites. As judged by 24-h LD₅₀ values, potent fumigant action was observed with clove bud oil （12.20 μg/cm²）, showing an adequate acaricidal activity against indoor Dermatophagoides farinae.

Key words: Clove bud oil, Dermatophagoides farinae, Acaricide