›› 2006, Vol. 24 ›› Issue (5): 9-359.

• 实验研究 • Previous Articles     Next Articles

Construction and Screening of Recombinant Fowlpox Virus ExpressingEimeria tenella F2 Hybrid Strain SO7 Gene

YANG Gui-lian1,2;ZHANG Xi-chen1;ZHAO Quan2;LI Jian-hua1;YIN Ji-gang1   

  1. 1 College of Animal Science and Veterinary Medicine，Jilin University，Changchun 130062，China；2 College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-10-30 Published:2006-10-30
  • Contact: ZHANG Xi-chen

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Abstract: Objective To Construct the recombinant fowlpox virus expressing Eimeria tenella F2 hybrid strain SO7 gene. Methods A recombinant expression plasmid pUTA-SO7 was constructed by inserting the SO7 gene of Eimeria tenella F2 hybrid strain into downstream of a hybrid poxvirus promoter which was flanked by the TK gene of fowlpox virus（FPV）. The constructed pUTA-SO7 was firstly transfected into chicken embryo fibroblast cells（CEF） pre-infected with FPV strain 282E4 by using liposome， then the viruses resulted from the transfection were selected for 2 passages by culturing in CEF cells with MEM medium containing 40 mg/L 5-bromo-2-deoxy-uridine（BrdU）. The selected viruses were plaque-purified in CEF cultured with MEM medium without BrdU. Results Polymerase chain reaction （PCR）， indirect immunofluorescence assay and Western blotting showed that SO7 gene was expressed in recombinant fowlpox virus. Conclusion The recom-binant FPV （rFPV） expressing the SO7 gene has been obtained.

Key words: Eimeria tenella, SO7, Recombinant, Fowlpox virus