›› 2006, Vol. 24 ›› Issue (2): 7-113.

• 论著 • Previous Articles     Next Articles

A Comparative Study on Isoenzymes of Sarcocystis spp. fromCattle and Water Buffaloes

LIANG Yi-long1,2;XIE Yong-fang1,2;ZUO Yang-xian1;TAN De-yong;SHU Kun-xian;CHEN Xin-wen   

  1. 1 Bioinformatics College, Chongqing University of Posts and Communications, Chongqing 400065, China；2 Biollogy College, Yunnan University, Kunming 650091, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-04-30 Published:2006-04-30

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Abstract: Objective To analyze the zymogram of peroxidase （PER） and phosphoglucose isomerase （PGI） of three species of Sarcocystis. Methods The collected parasites were homogenized and fragmented by ultrasonication. After centrifugation, the supernatants were analyzed by isoelectric focusing electrophoresis. Results The isolates of S. cruzi from infected water buffalo and cattle all showed identical enzyme profiles, 7 bands of PER at pH4.44~6.98 and 6 bands of PGI at pH 4.66~6.53；and same with the isolates of S. hirsuta. 5 bands of PER at pH 4.97~7.15 and 4 bands of PGI at pH 4.70~6.51. The zymograms among S. cruzi, S. hirsuta and S. fusiformis were different considerably. Conclusion The data support the hypothesis that both water buffalo and cattle are the natural intermediate hosts of S. cruzi and S. hirsuta at the gene level. S. cruzi, S. hirsuta and S. fusiformis are different species.

Key words: Sarcocystis, Isoenzyme, Isoelectric focusing, Gel electrophoresis