Abstract: Objective To establish a method for co-culture of amoebae and endosymbionts, also for continuously observing the microphenotype of amoebae. Methods 24 wells culture plate with cover glass on the wells was used as containers. Amoebae and Candida albicans were co-cultured in microdrop of medium in the wells at 37℃, and observed under ×1000. Results Continuous observation revealed trophozoites in various shapes like letters T, K, or Y, their movement and ingestion phenomenon were observed. Conclusion The micro-culture method is useful in observing the amoebal morphology and its phagocytic process to Candida albicans.

Key words: Free-living amoeba, Candida albicans, Morphology of living object, Micro-culture