Screening and Expression of Recombinant Human Monoclonal Antibody Fab Fragments Specific to Entamoeba histolytica

Abstract

Abstract: Objective To prepare recombinant human monoclonal antibody Fab fragments specific to the surface antigen of Entamoeba histolytica. Methods Total RNA was isolated from lymphocytes which were separated from an asymptomatic E. histolytica cyst carrier. The genes of IgG light chain and Fd region of heavy chain were amplified by a reverse transcriptase PCR and ligated with a plasmid vector. After the genes were introduced into Escherichia coli, the clones expressing Fab fragments specific to the surface antigen of E. histolytica were screened and the product was purified. Results Thirty thousand clones were screened and one of them was proved positive to the surface antigen of E. histolytica. Conclusion This study demonstrated that the bacterial system can be used to produce recombinant human monoclonal antibody Fab fragments specific to the surface antigen of E. histolytica and they may be applicable for the future diagnosis and treatment of the infection.

Key words: Entamoeba histolytica, monoclonal antibodies, recombinant DNA, gene expression

SHUQing;SHAOHong-xia;HiroshiTachibana;CHENGXun-jia. Screening and Expression of Recombinant Human Monoclonal Antibody Fab Fragments Specific to Entamoeba histolytica[J]. , 2004, 22(2): 1-69.

[1]	SU Ya-jing, QIAO Xia, WANG Peng-tao, KANG Yu-ting, YANG Ning-ai, JIA Wei, ZHAO Zhi-jun. Gene expression profiling of human lung adenocarcinoma of A549 cells induced by Toxoplasma virulence-related effector ROP16Ⅲ [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2021, 39(4): 473-480.
[2]	Gang CHENG, Qian-bo WANG, qiang GONG, De-hui XIONG. Comparative expression of HSP90α and KPNA2 genes in different tissues of Microtus fortis and their effects on the resistance against Schistosoma japonicum infection in recipient mice [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(5): 552-556.
[3]	Guang-yao MAO, Yun-hai GUO, Yi ZHANG, Ning XIAO. Effects of Cu²⁺ stress on the expression of metallothionein mRNA in Pomacea canaliculata [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(3): 375-378.
[4]	Guang-yao MAO, Hong-rang ZHOU, Yun HUANG, Zhi-yuan YUE, Lei DUAN, Qiu-li XU, Yun-hai GUO, Zhi-sheng DANG, Yi ZHANG, Ning XIAO. Analysis of glutathione S-transferase gene expression in Pomacea canaliculata under Cu²⁺ stress [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(2): 213-218.
[5]	Zhi-yuan YUE, Yun-hai GUO, Zhi-qiang QIN, Yi ZHANG. Research progress on snail gene expression associated with parasite infections [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2019, 37(1): 92-96.
[6]	WANG Nan1, TENG Fei-xiang1, YU Li-li1, YANG Li1, ZHANG Cheng-bo1, ZHOU Ying1,2, CUI Yu-bao1,2*. Cloning and expression of the full-length gene of group 8 allergen of Dermatophagoides farinae [J]. , 2017, 35(4): 8-351-356.
[7]	Ai-ping YU, Yu-juan SHEN. Research progress on long non-coding RNA in parasitology [J]. CHINESE JOURNAL OF PARASITOLOGY AND PARASITIC DISEASES, 2017, 35(3): 294-299.
[8]	JIA Bing-1, LIU Yu-Ming-2, WANG Qi-Yan-1, ZHANG Gong-Ling-1, WANG Jie-1, DAI Jia-Lin-1, HUANG Jiang-1*. Morphological Characteristics and Gene Expression of Chrysomya megacephala Eggs in Different Developmental Stages [J]. , 2014, 32(4): 10-295-298.
[9]	Zang-Wei, LU Wei-Yuan, XU Yu-Xin, CAO Jian-Ping. Cloning and Characterization of Conservative Region of Tyrosine Kinase 4, A Novel Gender-Associated Gene of Schistosoma japonicum [J]. , 2014, 32(3): 1-167-171.
[10]	CAI Hui-Xia, SHEN Yu-Juan, HAN Xiu-Min, YUAN Zhong-Ying, WANG Hu, XU Yu-Xin, HU Yuan, LU Wei-Yuan, GUAN Ya-Yi, CAO Jian-Ping. Cloning, Expression and Immunodiagnostic Evaluation of Antigen EPC1 from Echinococcus granulosus [J]. , 2011, 29(3): 2-167-171.
[11]	WANG Yi-hui，LI Ya-jie. Progress on Regulation of Gene Expression in Giardia lamblia* [J]. , 2011, 29(2): 12-138-141.
[12]	KANGJin-feng;HUHan-hua;Baishanbieke;CHENRong;Abulizi. In vitro* Observation on the Apoptosis Induced by H₂O₂ in Protoscolex of Echinococcus granulosus [J]. , 2008, 26(5): 3-337.
[13]	QUANHong;TANGLin-hua. In vitro* Potentiation of Chloroquine Activity in Plasmodium falciparum by Ketotifen and Cyproheptadine [J]. , 2008, 26(5): 4-342.
[14]	CHENJia-jie;XIALi-xin;LIUZhi-gang;LIUWen;JIKun-mei. Cloning，Expression and Purification of Allergen Arginine Kinase from Periplaneta americana* and its Allergic Activity [J]. , 2008, 26(5): 8-360.
[15]	CHENQin;LIANGWan-qi;QIANBing-jun;SHENHui-feng;CAOJian-ping;XUYu-xin;ZHANGDa-bing;TANGLin-hua*. Introduction of Plasmodium falciparum C－terminal Region ofthe Merozoite Surface Protein Gene into the Chloroplast of Tobacco [J]. , 2008, 26(4): 5-267.

Screening and Expression of Recombinant Human Monoclonal Antibody Fab Fragments Specific to Entamoeba histolytica

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments