Abstract: 　Objective To analyze the pooled sampling method for estimating the vector prevalence theoretically and provide the pooling schemes. Methods The criterion of mean square error (MSE) was used to determine the pool size and the number of pools. Results In comparison with the traditional method based upon testing samples one by one, the pooling method suggested in the present paper greatly reduced the laborious work load. Conclusion Based on the criterion of MSE and the predicted vector infection rate, the method was proved feasible in determining pool size and number of pools. The estimator x/mn is more manageable and precise than the traditional unbiased estimator.

Key words: sporozoite rate, pooled sampling, parameter estimation, criterion of MSE