›› 2001, Vol. 19 ›› Issue (2): 10-99.

• 论著 • Previous Articles     Next Articles

Application of Dot Immunogold Filtration Assay for Detecting Serum Antibodies in Clonorchiasis Patients

LIU Deng-yu 1;HU Wen-qing 1;ZHANG Hong-man 2   

  1. 1 Department of Parasitology;Guangxi Medical University;Nanning 530021; 2 Institute of Parasitic Diseases Control of Guangxi Zhuang Autonomous Region;Nanning 530021
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-04-30 Published:2001-04-30

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Abstract: 　Objective To establish a new, fast,sensitive and specific assay for detecting antibodies in clonorchiasis.Methods Using Clonorchis sinensis adult worm antigen and SPA labelled with colloidal gold as color developing agent,a dot immunogold filtration assay (DIGFA) for detecting clonorchiasis antibodies was tested. Dot-ELISA was used as the parallel control.Results The positive rate of DIGFA and dot-ELISA in testing the sera from 119 proved cases were 96.4%(115/119) and 92.4%(110/119), respectively,the difference between the two assays being in significant ( P >0.05). The negative rate of DIGFA in healthy people was 100%(40/40). The cross reaction rate in 20 cysticercosis cases and 25 schistosomiasis cases were 5%(1/20) and 4%(1/25), respectively. Both coincidence rates comparing DIGFA with dot-ELISA were 90.9%(50/55). Conclusion DIGFA is as sensitive and specific as the dot-ELISA,and has the advantages of simplicity and without specific equipment.

Key words: clonorchisasis, dot immunogold filtration assay, dot ELISA