›› 2000, Vol. 18 ›› Issue (6): 1-324.

• 论著 •     Next Articles

SEQUENCE ANALYSIS OF SSU rDNA VARIABLE REGIONS OF LEISHMANIAISOLATES FROM HILLY FOCI AND PLAIN FOCI OF CHINA

BU Ling-yi 1;HU Xiao-su 1;JING Bao-qian 2;YI Tao-lin 1
  

  1. 1 Department of Parasitology;West China University of Medical Sciences;Chengdu 610041; 2 Department of Microbiology;North Sichuan Medical College;Nanchong 637000
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-12-30 Published:2000-12-30

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Abstract:

　Objective] To analyze the sequence difference of the SSU rDNA variable regions of Leishmania isolates from hilly foci and plain foci of China. [Methods] Specific SSU rDNA fragments from nuclear DNA of five Leishmania species and isolates were amplified by PCR. The amplified DNA fragments were cloned into pGEM R-T Easy vector. The specific fragments were sequenced by the automated DNA sequencer. [Results] Sequence analysis showed that the amplified DNA fragments of five Leishmania species and isolates were all 392 bp in length, point mutations were located in the two unique sequence (UQ-Ⅰ and UQ-Ⅱ); L.d.SC10 and L.d.GS7 had two same point mutations in UQ-Ⅱ, only L.d.GS7 had one in UQ-Ⅰ; no insertion/deletion. [Conclusion] Sequence difference of the SSU rDNA variable region existed between Leishmania isolates from hilly foci and plain foci; The sequences of the SSU rDNA variable regions of L.d. SD2 isolate and L.infantum were identical.

Key words: Leishmania, small subunit ribosomal DNA, polymerase chain reaction, cloning, sequence analysis, genetic variation