Abstract: 　Objective] To identifiy the etiologic agent from a paragonimiasis patient using molecular techniques. [Methosd] The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product . ITS2 genes from eggs of Paragonimus westermani and Pagumogonimus skrjabini (both from animal hosts) were also sequenced for comparison. In addition , morphological comparisons were made with the eggs of the two species. [Results] The ITS2 gene from the human case was 100% identical with the sequence from the eggs of P westermani from an experimentally infected dog but only 92% identical with the sequence from the eggs of P skrjabini. Morphologically, the eggs from the human case more resembled those from P westermani infected dog. [Conclunsion] The patient was diagnosd to be suffered from paragonimiasis westermani by gene sequence analysis.

Key words: paragonimiasis westermani, nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene